Literature summary for 2.7.7.B22 extracted from

Carpentier, C.E.; Schreifels, J.M.; Aronovich, E.L.; Carlson, D.F.; Hackett, P.B.; Nesmelova, I.V.
NMR structural analysis of Sleeping Beauty transposase binding to DNA (2014), Protein Sci., 23, 23-33.

Search for more literature for 2.7.7.B22

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Homo sapiens	5634	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the sleeping beauty transposon DNA contains the gene to be inserted into the target DNA flanked by inverted terminal repeats (IR-DRleft and IR-DRright). Each inverted terminal repeat contains two, inner and outer, direct repeats (DRs). The DRs represent binding sites for the transposase enzyme. Sleeping beauty, SB, appears to be the most random in preferences for integration sites, requiring only a TA dinucleotide basepair. Binding mechanism, detailed overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the sleeping beauty transposon DNA contains the gene to be inserted into the target DNA flanked by inverted terminal repeats (IR-DRleft and IR-DRright). Each inverted terminal repeat contains two, inner and outer, direct repeats (DRs). The DRs represent binding sites for the transposase enzyme. Sleeping beauty, SB, appears to be the most random in preferences for integration sites, requiring only a TA dinucleotide basepair. Binding mechanism, detailed overview	Homo sapiens	?	-	?
additional information	interaction analysis of full-length enzyme and of PAI subdomain with transposon DNA, overview	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	domain structure of SB transposase enzyme, and solution conformation of full-length DNA-binding domain of SB transposase, [15N,1H]-HSQC spectra, overview. The DNA-binding domain is predicted to have two subdomains, PAI and RED, containing three alpha-helices. Residues 97-123 represent a nuclear localization signal (NLS). The catalytic domain contains three conserved catalytic residues, the DDE motif. NMR solution structure of the PAI subdomain, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
SB transposase	-	Homo sapiens
sleeping beauty transposase	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	the SB transposon is the most active DNA transposon in vertebrate animal cells	Homo sapiens

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Release 2023.1 (January 2023)