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Literature summary for 2.7.7.B22 extracted from

  • Ellis, M.J.; Trussler, R.S.; Haniford, D.B.
    Hfq binds directly to the ribosome-binding site of IS10 transposase mRNA to inhibit translation (2015), Mol. Microbiol., 96, 633-650.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.B22

Cloned(Commentary)

Cloned (Comment) Organism
gene EC990741_2310, TN10 includes genes jemA, jemB, jemC, tetR, tetA, tetC, and tetD and the left and right genes IS10L and IS10R, IS10R encodes a functional transposase protein that catalyzes the chemical steps in Tn10/IS10 transposition Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 IS10R
-

Subunits

Subunits Comment Organism
homodimer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
IS10 transposase
-
 Escherichia coli

Expression

Organism Comment Expression
Escherichia coli Hfq represses IS10/Tn10 transposase expression through both antisense RNA-dependent and independent mechanisms. Hfq binds directly to the ribosome-binding site of IS10 transposase mRNA to inhibit translation, Hfq binding to site 1 is critical for regulation of transposase expression in vivo. An interaction between the distal surface of Hfq and the ribosome-binding site of transposase mRNA (RNA-IN) is required for repressing translation initiation. Hfq represses transposase translation by facilitating antisense pairing as well as through an antisense-independent mechanism, overview. 3.2Fold decrease in transposase expression in the presence versus the absence of Hfq down
Escherichia coli impact of mutant forms of Hfq on IS10 transposase expression and transposition, Hfq binding of native RNA-IN in vivo is inhibited by site 1 mutations, overview additional information
Escherichia coli the small RNA ChiX activates transposase expression by titrating Hfq away from transposase mRNA, RNA-IN. Overexpression of ChiX results in a 4.5fold reduction in the amount of RNA-IN associated with Hfq. ChiX positively regulates IS10 transposase translation. ChiX competes with RNA-IN for Hfq-binding in vivo up

General Information

General Information Comment Organism
metabolism Hfq represses IS10/Tn10 transposase expression through both antisense RNA-dependent and independent mechanisms. Hfq binds directly to the ribosome-binding site of IS10 transposase mRNA to inhibit translation, regulatory role of Hfq in the absence of the IS10 antisense RNA. The interaction is critical for the in vivo association of Hfq and RNA-IN. Hfq is a critical component of post-transcriptional regulatory networks in most bacteria Escherichia coli
physiological function IS10R encodes a functional transposase protein that catalyzes the chemical steps in Tn10/IS10 transposition. In addition to transposase mRNA (RNA-IN), IS10 encodes an asRNA (RNA-OUT) that represses transposase translation by blocking ribosome binding Escherichia coli