Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.B16 extracted from

  • Liu, L.; Komori, K.; Ishino, S.; Bocquier, A.A.; Cann, I.K.; Kohda, D.; Ishino, Y.
    The archaeal DNA primase: biochemical characterization of the p41-p46 complex from Pyrococcus furiosus (2001), J. Biol. Chem., 276, 45484-45490.
    View publication on PubMed
Search for more literature for 2.7.7.B16

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the gene for the p58-like protein (gene product: Pfup46), expression in Escherichia coli Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40772
-
 1 * 46209 (large subunit Pfup46) + 1 * 40772 (small subunit Pfup41), calculated from sequence Pyrococcus furiosus
46209
-
 1 * 46209 (large subunit Pfup46) + 1 * 40772 (small subunit Pfup41), calculated from sequence Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NTP + n NTP Pyrococcus furiosus the p41-p46 complex synthesizes the primers, which can be extended in the combination with other replication proteins from Pyrococcus furiosus N(pN)n + n diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q9P9H1 and Q8U4H7 Q9P9H1: small subunit, Q8U4H7: large subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dNTP + n dNTP the p41-p46 primase preferentially uses dNTPs compared to NTPs. DNA strand synthesis is stimulated by the addition of NTPs or by addition of ATP to the same level. ATP is the preferable initiating nucleotide for the p41-p46 complex Pyrococcus furiosus dN(pdN)n + n diphosphate
-
 ?
NTP + n NTP the p41-p46 complex synthesizes the primers, which can be extended in the combination with other replication proteins from Pyrococcus furiosus Pyrococcus furiosus N(pN)n + n diphosphate
-
 ?
NTP + n NTP the p41-p46 primase preferentially uses dNTPs compared to NTPs. The primase complex synthesizes RNA segments only when NTPs but not dNTPs are added as the substrates. When both dNTPs and NTPs exist at equal concentrations, no RNA products are observed. ATP is the preferable initiating nucleotide for the p41-p46 complex Pyrococcus furiosus N(pN)n + n diphosphate
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 46209 (large subunit Pfup46) + 1 * 40772 (small subunit Pfup41), calculated from sequence Pyrococcus furiosus
More the Pfup46 protein increases the affinity of the primase to DNA by forming a complex with the catalytic Pfup41 subunit Pyrococcus furiosus