Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Streptomyces antibioticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxy-4-methylanthranilate | Streptomyces antibioticus | - |
diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces antibioticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, phenyl Sepharose column chromatography, QAE-Sepharose column chromatography, and Sephacryl gel filtration | Streptomyces antibioticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Streptomyces antibioticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0008 | - |
crude enzyme, at pH 7.5 and 37°C | Streptomyces antibioticus |
0.795 | - |
after 958fold purification, at pH 7.5 and 37°C | Streptomyces antibioticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxy-4-methylanthranilate | - |
Streptomyces antibioticus | diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? | |
ATP + 3-hydroxyanthranilate | - |
Streptomyces antibioticus | ? | - |
? | |
ATP + 3-hydroxybenzoate | - |
Streptomyces antibioticus | ? | - |
? | |
ATP + anthranilate | - |
Streptomyces antibioticus | ? | - |
? | |
ATP + benzoate | - |
Streptomyces antibioticus | ? | - |
? | |
ATP + glycine | low activity | Streptomyces antibioticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Streptomyces antibioticus |
Synonyms | Comment | Organism |
---|---|---|
ACMSI | - |
Streptomyces antibioticus |
actinomycin synthetase I | - |
Streptomyces antibioticus |