Cloned (Comment) | Organism |
---|---|
His-tag version expressed in Escherichia coli BL21 (DE3) | Hordeum vulgare |
Protein Variants | Comment | Organism |
---|---|---|
Ccut-101 | 101 amino acid residues deleted (exons 16-19, and 1 amino acid residue of exon 15) | Hordeum vulgare |
Ccut-32 | 32 amino acid residues deleted (exon 18 and exon 19) | Hordeum vulgare |
Ccut-67 | 67 amino acid residues deleted (exons 17-19) | Hordeum vulgare |
Ccut-8 | 8 amino acid residues deleted (last exon (exon 19)) | Hordeum vulgare |
Delta 1-4 | deletion of 4 amino acid residues of the C-terminal domain, possibly involved in oligomerization | Hordeum vulgare |
Delta 1-8 | deletion of 8 amino acid residues of the C-terminal domain, possibly involved in oligomerization | Hordeum vulgare |
Delta-NB | deletion mutant: amino acid residue 96-100 deleted (essential for catalysis) | Hordeum vulgare |
K183A | possibly involved in subunit interaction | Hordeum vulgare |
K260A | possibly involved in diphosphate binding | Hordeum vulgare |
K332A | possibly involved in subunit interaction | Hordeum vulgare |
K405A | possibly involved in subunit interaction | Hordeum vulgare |
Ncut-21 | 21 amino acid residues deleted (exon 1) | Hordeum vulgare |
Ncut-37 | 37 amino acid residues deleted (exon 1 and exon 2) | Hordeum vulgare |
Y192A | possibly involved in UDP-glucose binding | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
diphosphate | Y192A mutant protein | Hordeum vulgare | |
0.03 | - |
diphosphate | Ccut-8 mutant protein | Hordeum vulgare | |
0.03 | - |
UDP-glucose | Ccut-8 mutant protein | Hordeum vulgare | |
0.03 | - |
diphosphate | K183A mutant protein | Hordeum vulgare | |
0.03 | - |
UDP-glucose | K183A mutant protein | Hordeum vulgare | |
0.03 | - |
UDP-glucose | K332A mutant protein | Hordeum vulgare | |
0.031 | - |
UDP-glucose | Y192A mutant protein | Hordeum vulgare | |
0.034 | - |
UDP-glucose | wild-type protein | Hordeum vulgare | |
0.039 | - |
diphosphate | wild-type protein | Hordeum vulgare | |
0.04 | - |
diphosphate | K332A mutant protein | Hordeum vulgare | |
0.04 | - |
UDP-glucose | K405A mutant protein | Hordeum vulgare | |
0.074 | - |
UDP-glucose | K260A mutant protein | Hordeum vulgare | |
0.09 | - |
diphosphate | Ncut-21 mutant protein | Hordeum vulgare | |
0.118 | - |
UDP-glucose | Ccut-101 mutant protein | Hordeum vulgare | |
0.24 | - |
diphosphate | K405A mutant protein | Hordeum vulgare | |
0.31 | - |
UDP-glucose | Ccut-32 mutant protein | Hordeum vulgare | |
0.362 | - |
UDP-glucose | Ccut-67 mutant protein | Hordeum vulgare | |
0.503 | - |
UDP-glucose | Delta 1-8 mutant protein | Hordeum vulgare | |
0.555 | - |
UDP-glucose | Delta 1-4 mutant protein | Hordeum vulgare | |
0.9 | - |
diphosphate | Ncut-37 mutant protein | Hordeum vulgare | |
1.1 | - |
UDP-glucose | Ncut-21 mutant protein | Hordeum vulgare | |
4.9 | - |
diphosphate | K260A mutant protein | Hordeum vulgare | |
6.4 | - |
diphosphate | Ccut-32 mutant protein | Hordeum vulgare | |
9.8 | - |
diphosphate | Ccut-67 mutant protein | Hordeum vulgare | |
29.4 | - |
diphosphate | Delta 1-4 mutant protein | Hordeum vulgare | |
33.3 | - |
diphosphate | Ccut-101 mutant protein | Hordeum vulgare | |
57 | - |
UDP-glucose | Ncut-37 mutant protein | Hordeum vulgare | |
60 | - |
diphosphate | Delta 1-8 mutant protein | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q43772 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography | Hordeum vulgare |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
- |
- |
Delta-NB mutant protein | Hordeum vulgare |
0.02 | - |
Ncut-37 mutant protein | Hordeum vulgare |
0.21 | - |
Ccut-101 mutant protein | Hordeum vulgare |
0.27 | - |
Ccut-32 mutant protein | Hordeum vulgare |
0.42 | - |
K260A mutant protein | Hordeum vulgare |
2.1 | - |
Delta 1-4 mutant protein | Hordeum vulgare |
3.1 | - |
Ccut-67 mutant protein | Hordeum vulgare |
5.4 | - |
Delta 1-8 mutant protein | Hordeum vulgare |
12.8 | - |
Ncut-21 mutant protein | Hordeum vulgare |
591 | - |
Y192A mutant protein | Hordeum vulgare |
752 | - |
K183A mutant protein | Hordeum vulgare |
764 | - |
K405A mutant protein | Hordeum vulgare |
853 | - |
K332A mutant protein | Hordeum vulgare |
1074 | - |
wild-type protein | Hordeum vulgare |
1547 | - |
Ccut-8 mutant protein | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | - |
Hordeum vulgare | diphosphate + UDP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 52000-53000, SDS-PAGE | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose pyrophosphorylase | - |
Hordeum vulgare |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hordeum vulgare | K183A mutant protein | - |
5.47 |
Hordeum vulgare | K332A mutant protein | - |
5.47 |
Hordeum vulgare | K405A mutant protein | - |
5.47 |
Hordeum vulgare | Ncut-21 mutant protein | - |
5.54 |
Hordeum vulgare | Delta 1-8 mutant protein | - |
5.55 |
Hordeum vulgare | Delta-NB mutant protein | - |
5.55 |
Hordeum vulgare | wild-type protein | - |
5.55 |
Hordeum vulgare | Y192A mutant protein | - |
5.55 |
Hordeum vulgare | Ccut-101 mutant protein | - |
5.58 |
Hordeum vulgare | Delta 1-4 mutant protein | - |
5.63 |
Hordeum vulgare | Ccut-67 mutant protein | - |
5.69 |
Hordeum vulgare | Ncut-37 mutant protein | - |
5.7 |
Hordeum vulgare | Ccut-32 mutant protein | - |
5.79 |
Hordeum vulgare | Ccut-8 mutant protein | - |
5.8 |