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Literature summary for 2.7.7.9 extracted from
- Uridine diphosphate glucose pyrophosphorylase of Acanthamoeba castellanii. Purification, kinetic, and developmental studies (1974), J. Biol. Chem., 249, 7832-7840.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | product inhibition | Acanthamoeba castellanii |  |
| UTP | product inhibition | Acanthamoeba castellanii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | 0.066 | UDP-glucose | pH 7.6, 37°C | Acanthamoeba castellanii | |
| 0.058 | 0.07 | UTP | pH 8.0, 37°C | Acanthamoeba castellanii | |
| 0.14 | 0.18 | alpha-D-glucose 1-phosphate | - |
Acanthamoeba castellanii | |
| 0.68 | 1 | alpha-D-glucose 1-phosphate | pH 8.0, 37°C | Acanthamoeba castellanii | |
| 2.4 | - |
diphosphate | pH 7.6, 37°C | Acanthamoeba castellanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Acanthamoeba castellanii | |
| Co2+ | activation | Acanthamoeba castellanii | |
| Mg2+ | requirement | Acanthamoeba castellanii | |
| Mg2+ | MgUTP is the actual substrate | Acanthamoeba castellanii | |
| Mg2+ | Mg-diphosphate is the actual substrate | Acanthamoeba castellanii | |
| additional information | requirement for divalent cations | Acanthamoeba castellanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | Acanthamoeba castellanii | enzyme is induced during amoeba encystment | diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | Acanthamoeba castellanii NEFF | enzyme is induced during amoeba encystment | diphosphate + UDP-glucose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthamoeba castellanii | - |
Neff | - |
| Acanthamoeba castellanii NEFF | - |
Neff | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acanthamoeba castellanii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cyst | - |
Acanthamoeba castellanii | - |
| cyst | enzyme activity increases during encystment | Acanthamoeba castellanii | - |
| trophozoite | - |
Acanthamoeba castellanii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.8 | - |
purified enzyme | Acanthamoeba castellanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + CDP-glucose | no activity | Acanthamoeba castellanii | CTP + alpha-D-glucose 1-phosphate | - |
? | |
| diphosphate + GDP-glucose | no activity | Acanthamoeba castellanii | GTP + alpha-D-glucose 1-phosphate | - |
? | |
| diphosphate + TDP-glucose | no activity | Acanthamoeba castellanii | TTP + alpha-D-glucose 1-phosphate | - |
? | |
| additional information | no activity with ADP-glucose | Acanthamoeba castellanii | ? | - |
? | |
| additional information | no activity with ATP, CTP, and GTP | Acanthamoeba castellanii | ? | - |
? | |
| additional information | no activity with ADP-glucose | Acanthamoeba castellanii NEFF | ? | - |
? | |
| additional information | no activity with ATP, CTP, and GTP | Acanthamoeba castellanii NEFF | ? | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | highly specific for UTP | Acanthamoeba castellanii | diphosphate + UDP-glucose | specific for UDP-glucose | r | |
| UTP + alpha-D-glucose 1-phosphate | reverse reaction: highly specific for UDP-glucose | Acanthamoeba castellanii | diphosphate + UDP-glucose | specific for UDP-glucose | r | |
| UTP + alpha-D-glucose 1-phosphate | enzyme is induced during amoeba encystment | Acanthamoeba castellanii | diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | highly specific for UTP | Acanthamoeba castellanii NEFF | diphosphate + UDP-glucose | specific for UDP-glucose | r | |
| UTP + alpha-D-glucose 1-phosphate | reverse reaction: highly specific for UDP-glucose | Acanthamoeba castellanii NEFF | diphosphate + UDP-glucose | specific for UDP-glucose | r | |
| UTP + alpha-D-glucose 1-phosphate | enzyme is induced during amoeba encystment | Acanthamoeba castellanii NEFF | diphosphate + UDP-glucose | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Acanthamoeba castellanii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
30 min, stable | Acanthamoeba castellanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 7.6 | pyrophosphorolysis | Acanthamoeba castellanii |
| 8 | 9 | UDP-glucose synthesis | Acanthamoeba castellanii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9.8 | about half-maximal activity at pH 7.2 and about 90% of maximal activity at pH 9.8 | Acanthamoeba castellanii |
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