Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | activation | Escherichia coli | |
adenosine | activation by nucleotides, most efficient in presence of two different nucleotides | Escherichia coli | |
GTP | activation by nucleotides, most efficient in presence of two different nucleotides | Escherichia coli | |
ITP | activation by nucleotides, most efficient in presence of two different nucleotides | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Escherichia coli | |
glutamate | - |
Escherichia coli | |
glutamine | - |
Escherichia coli | |
SO42- | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
Adenylyl-[L-glutamate:ammonia ligase (ADP-forming)] | calculated as adenylyl groups removed from substrate | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
arsenate | activation | Escherichia coli | |
Mg2+ | activation | Escherichia coli | |
Mn2+ | activation | Escherichia coli | |
additional information | not activated by Ca2+, Cd2+, Zn2+, Ba2+ | Escherichia coli | |
phosphate | activation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl-[L-glutamate:ammonia ligase (ADP-forming)] + H2O | Escherichia coli | activation of adenylated glutamine synthetase, EC 6.3.1.2, which is less active in catalyzing glutamine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 2 weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl-[L-glutamate:ammonia ligase (ADP-forming)] + H2O | - |
Escherichia coli | adenylate + [L-glutamate:ammonia ligase (ADP-forming)] | the primary product may be ADP which is then degraded to AMP | ir | |
adenylyl-[L-glutamate:ammonia ligase (ADP-forming)] + H2O | activation of adenylated glutamine synthetase, EC 6.3.1.2, which is less active in catalyzing glutamine biosynthesis | Escherichia coli | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | 80% of maximal activity at pH 6.5, 50% of maximal activity at pH 8.0 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | activation by nucleotides, most efficient in presence of two different nucleotides | Escherichia coli | |
AMP | activation by nucleotides, most efficient in presence of two different nucleotides | Escherichia coli | |
ATP | activation by nucleotides, most efficient in presence of two different nucleotides | Escherichia coli | |
CTP | activation by nucleotides, most efficient in presence of two different nucleotides | Escherichia coli | |
UTP | activation by nucleotides, most efficient in presence of two different nucleotides | Escherichia coli |