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Literature summary for 2.7.7.88 extracted from
- Histidine-mediated RNA transfer to GDP for unique mRNA capping by vesicular stomatitis virus RNA polymerase (2010), Proc. Natl. Acad. Sci. USA, 107, 3463-3468 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf21 insect cells | vesicular stomatitis virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H1227 | inactive | vesicular stomatitis virus |
| R1221 | inactive | vesicular stomatitis virus |
| R1228 | inactive | vesicular stomatitis virus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-triphospho-mRNA + GDP | vesicular stomatitis virus | - |
diphosphate + guanosine 5'-triphospho-mRNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| vesicular stomatitis virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose affinity resin chromatography | vesicular stomatitis virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-triphospho-mRNA + GDP | - |
vesicular stomatitis virus | diphosphate + guanosine 5'-triphospho-mRNA | - |
? | |
| additional information | free 5'-phosphorylated mRNA is completely inert as a substrate | vesicular stomatitis virus | ? | - |
? | |
| additional information | the enzyme is capable of transferring a polyribonucleotidyl group to a substrate using a catalytic histidine residue. The histidine-arginine motif is required for the enzyme activity at the step of the enzyme-5'-phosphorylated mRNA intermediate formation | vesicular stomatitis virus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L protein | - |
vesicular stomatitis virus |
| L-pRNA complex | - |
vesicular stomatitis virus |
| PRNTase | - |
vesicular stomatitis virus |
| RNA:GDP polyribonucleotidyltransferase | - |
vesicular stomatitis virus |
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Release 2023.1 (January 2023)
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