Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dsDNA | presence of dsDNA activates cGAS. dsDNA of 12 bp in length does not activate mcGAS efficiently, dsDNA of 18 bp in length has about 90% activity compared to salmon sperm DNA. Interactions of both DNA binding aites are essential for activation. The two sites bind cooperatively and site B plays a crucial role in binding. DNA binding by induces higher-order complex formation in solution | Homo sapiens | |
dsDNA | presence of dsDNA activates cGAS. dsDNA of 12 bp in length does not activate mcGAS efficiently, dsDNA of 18 bp in length has about 90% activity compared to salmon sperm DNA. Interactions of both DNA binding aites are essential for activation. The two sites bind cooperatively and site B plays a crucial role in binding. DNA binding by induces higher-order complex formation in solution | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
structure of cGAS catalytic domain | Homo sapiens |
structure of mouse cGAS bound to an 18 bp dsDNA. cGAS interacts with dsDNA through two binding sites, forming a 2:2 complex. enzyme forms a dimer in the crystal | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K160E | mutation dramatically reduces enzyme activity but only slightly reduces DNA binding | Mus musculus |
K240E | mutation dramatically reduces DNA binding, mutant still exhibits some catalytic activity | Mus musculus |
R158E | mutation dramatically reduces enzyme activity but only slightly reduces DNA binding | Mus musculus |
R161E | mutation dramatically reduces enzyme activity but only slightly reduces DNA binding | Mus musculus |
R222E | mutation dramatically reduces DNA binding, mutant still exhibits some catalytic activity | Mus musculus |
S165E | mutation dramatically reduces enzyme activity but only slightly reduces DNA binding | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8N884 | - |
- |
Mus musculus | Q8C6L5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | - |
Homo sapiens | 2 diphosphate + cyclic Gp(2'-5')Ap(3'-5') | product is cyclic Gp(2'-5')Ap(3'-5') | ? | |
ATP + GTP | - |
Mus musculus | 2 diphosphate + cyclic Gp(2'-5')Ap(3'-5') | product is cyclic Gp(2'-5')Ap(3'-5') | ? |