Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.85 extracted from

  • Mehne, F.M.; Schroeder-Tittmann, K.; Eijlander, R.T.; Herzberg, C.; Hewitt, L.; Kaever, V.; Lewis, R.J.; Kuipers, O.P.; Tittmann, K.; Stuelke, J.
    Control of the diadenylate cyclase CdaS in Bacillus subtilis: an autoinhibitory domain limits cyclic di-AMP production (2014), J. Biol. Chem., 289, 21098-21107.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.85

Protein Variants

Protein Variants Comment Organism
A61V 11.6fold increase in cyclic di-AMP production Bacillus subtilis
A76V 4.3fold increase in cyclic di-AMP production Bacillus subtilis
E46K 19.5fold increase in cyclic di-AMP production Bacillus subtilis
L44F 90.5fold increase in cyclic di-AMP production Bacillus subtilis
additional information very low expression of CdaS as the single diadenylate cyclase results in the appearance of spontaneous suppressor mutations. Several of these mutations in the cdaS gene affect the N-terminal domain of CdaS. The corresponding CdaS mutant proteins exhibit a significantly increased enzymatic activity. Deletion of the first or both N-terminal domain alpha-helices results also in strongly increased activity. The mutations and the deletions of the N-terminal domain result in conformational changes that lead to highly increased enzymatic activity. Although the full-length CdaS protein forms hexamers, a truncated version with a deletion of the first N-terminal helix formed dimers with high enzyme activity Bacillus subtilis
P201Q 12.8fold increase in cyclic di-AMP production Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 and 37000 and 167000 Da, gel filtration Bacillus subtilis
23000
-
-
 Bacillus subtilis
37000
-
 and 167000 and 17000 Da, gel filtration Bacillus subtilis
167000
-
 and 37000 and 17000 Da, gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31854
-
-
Bacillus subtilis 168 O31854
-
-

Subunits

Subunits Comment Organism
dimer 2 * 23000, calculated, plus hexamer and monomer Bacillus subtilis
hexamer 6 * 23000, calculated, plus monomer and dimer Bacillus subtilis
monomer 1 * 23000, calculated, plus hexamer and dimer Bacillus subtilis