Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.85 extracted from

  • Witte, G.; Hartung, S.; Buettner, K.; Hopfner, K.P.
    Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates (2008), Mol. Cell, 30, 167-178.
    View publication on PubMed
Search for more literature for 2.7.7.85

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis
expression in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallisation of apoenzyme, apoenzyme + ATPgammaS, apoenzyme + cordycepin-triphosphate, determination of the crystal structure at 2.1 A Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42700
-
 2 * 42700 Thermotoga maritima
42900
-
 8 * 42900 Bacillus subtilis
300000
-
 gel filtration Bacillus subtilis
353500
-
 analytical ultracentrifugation Thermotoga maritima
370000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ATP Thermotoga maritima specific recognition of adenine in the active site pocked. The enzyme signals DNA structures that interfere with chromosome segregation cyclic di-3',5'-adenylate. The enzyme activity is unaffected by linear DNA or DNA ends but strongly suppressed by branched nucleic acids such as Holliday junctions 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P37573
-
-
Thermotoga maritima Q9WY43
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP specific recognition of adenine in the active site pocked. The enzyme signals DNA structures that interfere with chromosome segregation cyclic di-3',5'-adenylate. The enzyme activity is unaffected by linear DNA or DNA ends but strongly suppressed by branched nucleic acids such as Holliday junctions Thermotoga maritima 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?
2 ATP specific recognition of adenine in the active site pocked. The enzyme does not convert GTP to cyclic di-3',5'-guanylate Thermotoga maritima 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?

Subunits

Subunits Comment Organism
octamer 2 * 42700 Thermotoga maritima
octamer 8 * 42900 Bacillus subtilis

Synonyms

Synonyms Comment Organism
BsuDisA
-
 Bacillus subtilis
TmaDisA
-
 Thermotoga maritima

General Information

General Information Comment Organism
physiological function the enzyme signals DNA structures that interfere with chromosome segregation cyclic di-3',5'-adenylate. The enzyme activity is unaffected by linear DNA or DNA ends but strongly suppressed by branched nucleic acids such as Holliday junctions Thermotoga maritima