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Literature summary for 2.7.7.83 extracted from
- A 17-amino acid insert changes UDP-N-acetylhexosamine pyrophosphorylase specificity from UDP-GalNAc to UDP-GlcNAc (1998), J. Biol. Chem., 273, 27055-27057.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| GST fusion protein expressed in Escherichia coli JM109 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
N-acetyl-D-glucosamine 1-phosphate | GST-fusion protein, N-acetylglucosamine 1-phosphate, pH 7.5, 37°C | Homo sapiens |  |
| 0.32 | - |
N-acetyl-D-glucosamine 1-phosphate | GST-fusion protein, N-acetyl-D-glucosamine 1-phosphate, pH 7.5, 37°C | Homo sapiens | |
| 0.38 | - |
N-acetyl-D-galactosamine 1-phosphate | GST-fusion protein, N-acetyl-D-galactosamine 1-phosphate, pH 7.5, 37°C | Homo sapiens | |
| 1.3 | - |
N-acetyl-D-galactosamine 1-phosphate | GST-fusion protein, N-acetyl-D-galactosamine 1-phosphate, pH 7.5, 37°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000, SDS-PAGE | Homo sapiens |
| 64000 | - |
x * 64000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography (glutathione-Sepharose), GST part removed | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| testis | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.119 | - |
GST-fusion protein, N-acetyl-D-galactosamine 1-phosphate, GST protein shows 0.02 micromol/min/mg in the assay, pH 7.5, 37°C | Homo sapiens |
| 0.3 | - |
GST fusion removed, N-acetyl-D-galactosamine 1-phosphate, pH 7.5, 37°C | Homo sapiens |
| 0.525 | - |
GST-fusion protein, N-acetyl-D-glucosamine 1-phosphate, GST protein shows 0.033 micromol/min/mg in the assay, pH 7.5, 37°C | Homo sapiens |
| 0.887 | - |
GST-fusion protein, N-acetyl-D-glucosamine 1-phosphate, GST protein shows 0.033 micromol/min/mg in the assay, pH 7.5, 37°C | Homo sapiens |
| 1.342 | - |
GST-fusion protein, N-acetyl-D-galactosamine 1-phosphate, GST protein shows 0.02 micromol/min/mg in the assay, pH 7.5, 37°C | Homo sapiens |
| 5.873 | - |
GST fusion removed, N-acetyl-D-glucosamine 1-phosphate, pH 7.5, 37°C | Homo sapiens |
| 5.919 | - |
GST fusion removed, N-acetyl-D-glucosamine 1-phosphate, pH 7.5, 37°C | Homo sapiens |
| 14.9 | - |
GST fusion removed, N-acety-D-lgalactosamine 1-phosphate, pH 7.5, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + N-acetyl-D-galactosamine 1-phosphate | - |
Homo sapiens | diphosphate + UDP-N-acetyl-D-galactosamine | - |
r | |
| UTP + N-acetyl-D-glucosamine 1-phosphate | - |
Homo sapiens | diphosphate + UDP-N-acetyl-D-glucosamine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, SDS-PAGE | Homo sapiens |
| ? | x * 64000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-N-acetylhexosamine pyrophosphorylase AGX1 | isoform of AGX2 | Homo sapiens |
| UDP-N-acetylhexosamine pyrophosphorylase AGX2 | isoform of AGX1 | Homo sapiens |
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