KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
RNA(n) | pH 8.4, 37°C | Staphylococcus aureus | |
0.042 | - |
2',3'-cyclic RNAn | pH 8.4, 37°C | Staphylococcus aureus | |
0.156 | - |
3'-phosphorylated RNA(n) | pH 8.4, 37°C | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q7A5X7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cyclic RNAn + phosphate | - |
Staphylococcus aureus | RNA(n-1) + a nucleotide diphosphate | - |
? | |
3'-phosphorylated RNA(n) + phosphate | - |
Staphylococcus aureus | RNA(n-1) + a nucleotide diphosphate | enzyme inefficiently degrades 3'-phosphorylated RNA | ? | |
poly(A)(n-1) + ADP | - |
Staphylococcus aureus | poly(A)n + phosphate | - |
r | |
poly(A)n + phosphate | - |
Staphylococcus aureus | poly(A)(n-1) + ADP | - |
r | |
RNA(n) + phosphate | - |
Staphylococcus aureus | RNA(n-1) + a nucleotide diphosphate | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | the cvfA-deletion mutant phenotype showing decreased agr expression and hemolysin production, is suppressed by disrupting pnpA-encoding PNPase. Loss of the 3'- to 5'-exonuclease activity is required for suppression. CvfA protein hydrolyzes a 2',3'-cyclic phosphodiester bond at the RNA 3' terminus, producing RNA with a 3'-phosphate. Purified PNPase efficiently degrades RNA with 2',3'-cyclic phosphate at the 3' terminus (2',3'-cyclic RNA), but it inefficiently degrades 3'-phosphorylated RNA | Staphylococcus aureus |