F635A |
site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme |
Escherichia coli |
F635A/F638A/H650A |
site-directed mutagenesis, the mutant enzyme shows highly reduced activity and an increased RNA binding constant compared to the wild-type enzyme |
Escherichia coli |
F635R/F638R/H650R |
site-directed mutagenesis, the mutant enzyme shows reduced activity and an increased RNA binding constant compared to the wild-type enzyme |
Escherichia coli |
F638A |
site-directed mutagenesis, the mutant enzyme shows reduced activity and an increased RNA binding constant compared to the wild-type enzyme |
Escherichia coli |
G570C |
site-directed mutagenesis, the mutant enzyme shows highly reduced activity and an increased RNA binding constant compared to the wild-type enzyme |
Escherichia coli |
G570C/V679A |
site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme |
Escherichia coli |
H650A |
site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme |
Escherichia coli |
I555T |
site-directed mutagenesis, the mutant enzyme shows slightly reduced activity compared to the wild-type enzyme |
Escherichia coli |
I576A |
site-directed mutagenesis, the mutant enzyme shows reduced activity and an increased RNA binding constant compared to the wild-type enzyme |
Escherichia coli |
I576A/F638A |
site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme |
Escherichia coli |
I576T |
site-directed mutagenesis, the mutant enzyme shows reduced activity and an increased RNA binding constant compared to the wild-type enzyme |
Escherichia coli |
I576T/F638A |
site-directed mutagenesis, the mutant enzyme shows reduced activity and an increased RNA binding constant compared to the wild-type enzyme |
Escherichia coli |
I576T/T585A |
site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme |
Escherichia coli |
K571L |
site-directed mutagenesis, the mutant enzyme shows reduced activity and an increased RNA binding constant compared to the wild-type enzyme |
Escherichia coli |
K571Q |
site-directed mutagenesis, the mutant enzyme shows reduced activity and an increased RNA binding constant compared to the wild-type enzyme |
Escherichia coli |
additional information |
construction of domain deletion mutants DELTAKH DELTAS1, DELTAKH, and DELTAS1 |
Escherichia coli |