Cloned (Comment) | Organism |
---|---|
overexpression of gpsI gene encoding PNPase in Escherichia coli | Streptomyces antibioticus |
Crystallization (Comment) | Organism |
---|---|
protein solution mixed at 3/1 ratio with well solution consisting of 2 M ammonium sulfate, 100 mM Tris-HCl, pH 8.5, 5 mM dithiothreitol and 0.75 mM Na2AsO4, crystals are harvested in a so called mirror solution from equilibrated droplets with buffer replacing enzyme solution and no added Na2AsO4, crystals of native PNPase, a tungstate derivative and a seleno-methionyl derivative | Streptomyces antibioticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81132 | - |
x * 81132, electrospray mass spectrometry | Streptomyces antibioticus |
81133 | - |
x * 81133, deduced from nucleotide sequence | Streptomyces antibioticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces antibioticus | Q53597 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, HiTrap Q, HiFlow Phenyl-Sepharose | Streptomyces antibioticus |
Subunits | Comment | Organism |
---|---|---|
? | x * 81133, deduced from nucleotide sequence | Streptomyces antibioticus |
? | x * 81132, electrospray mass spectrometry | Streptomyces antibioticus |