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Literature summary for 2.7.7.8 extracted from

  • Simuth, J.; Zelinka, J.S.; Polek, B.
    Polynucleotide phosphorylase from Streptomyces aureofaciens: purification and properties (1975), Biochim. Biophys. Acta, 379, 397-407.
    View publication on PubMed
Search for more literature for 2.7.7.8

Activating Compound

Activating Compound Comment Organism Structure
cytidyldadenylate dinucleoside activates GDP polymerization Kitasatospora aureofaciens
KCl 400 mM, 5fold activation of ADP polymerization Kitasatospora aureofaciens
NaCl 400 mM, 4fold activation of ADP polymerization Kitasatospora aureofaciens
NH4+ 400 mM, 3 and 7fold activation of ADP polymerization and poly(A) phosphorolysis, respectively Kitasatospora aureofaciens

Inhibitors

Inhibitors Comment Organism Structure
chlortetracycline competitive inhibition of ADP polymerization Kitasatospora aureofaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 ADP approx. value, also for CDP and UDP polymerization Kitasatospora aureofaciens
1
-
 ADP pH 9.5, 37°C, approx. value Kitasatospora aureofaciens
1
-
 UDP pH 9.5, 37°C, approx. value Kitasatospora aureofaciens
1
-
 CDP pH 9.5, 37°C, approx. value Kitasatospora aureofaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
Co2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
Cu2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
K+
-
 Kitasatospora aureofaciens
Mg2+ activity depends on divalent cation, order of efficiency: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
Mn2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
Na+
-
 Kitasatospora aureofaciens
Zn2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 2 * 100000, SDS-PAGE in the presence of 2-mercaptoethanol Kitasatospora aureofaciens
210000
-
 low speed sedimentation equilibrium Kitasatospora aureofaciens

Organism

Organism UniProt Comment Textmining
Kitasatospora aureofaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polymin P, DEAE-cellulose, Sephadex G-150, DEAE-cellulose, Sephadex G-200 Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.8
-
-
 Kitasatospora aureofaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + ADP
-
 Kitasatospora aureofaciens poly(A)+1 + phosphate
-
 r
poly(C) + CDP
-
 Kitasatospora aureofaciens poly(C)+1 + phosphate
-
 r
poly(U)+ UDP
-
 Kitasatospora aureofaciens poly(U)+1 + phosphate
-
 r
poly(U)+1 + phosphate
-
 Kitasatospora aureofaciens poly(U) + ADP
-
 r
ribonucleoside 5'-diphosphate + phosphate
-
 Kitasatospora aureofaciens ribonucleoside 5'-diphosphate + phosphate exchange reaction ?
RNAn + a nucleoside diphosphate polymerization of CDP Kitasatospora aureofaciens RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate polymerization of UDP Kitasatospora aureofaciens RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate polymerization of ADP Kitasatospora aureofaciens RNAn+1 + phosphate
-
 ?
RNAn + UDP
-
 Kitasatospora aureofaciens RNAn+1 + phosphate
-
 r
RNAn+1 + phosphate phosphorolysis of poly(U) Kitasatospora aureofaciens RNAn + a nucleoside diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 100000, SDS-PAGE in the presence of 2-mercaptoethanol Kitasatospora aureofaciens