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Literature summary for 2.7.7.8 extracted from
- Further characterization of the polynucleotide phosphorylase of Micrococcus luteus (1975), Nucleic Acids Res., 2, 149-163.
General Stability
| General Stability | Organism |
|---|---|
| enzyme form I is highly susceptible to proteolytic degradation | Micrococcus luteus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | - |
x * 71000, enzyme form T, enzyme form I shows several bands of different molecular sizes, SDS-PAGE | Micrococcus luteus |
| 230000 | - |
enzyme form T, sedimentation equilibrium ultracentrifugation | Micrococcus luteus |
| 270000 | - |
enzyme form I, sedimentation equilibrium ultracentrifugation | Micrococcus luteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus luteus | - |
a primer-independent, i.e. form I enzyme, and a primer-dependent, i.e. form T enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phosphocellulose chromatography, enzyme forms I and T | Micrococcus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNAn + a nucleoside diphosphate | - |
Micrococcus luteus | RNAn+1 + phosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 71000, enzyme form T, enzyme form I shows several bands of different molecular sizes, SDS-PAGE | Micrococcus luteus |
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Release 2023.1 (January 2023)
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