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Literature summary for 2.7.7.8 extracted from
- Polynucleotide phosphorylase (1982), The Enzymes, 3rd. Ed. (Boyer, P. D. , ed. ), 15B, 517-553.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Basic polypeptide | peptide from Escherichia coli extract, enhances ADP-phosphate exchange | Geobacillus stearothermophilus | |
| poly-L-lysine | stimulation of poly(A) synthesis, phosphorolysis of poly(A) is inhibited | Clostridium perfringens |  |
| Polyarginine | stimulation of poly(A) synthesis, phosphorolysis of poly(A) is inhibited | Clostridium perfringens | |
| Polyornithine | stimulation of poly(A) synthesis, phosphorolysis of poly(A) is inhibited | Clostridium perfringens | |
| spermidine | - |
Nicotiana tabacum | |
| spermidine | 0.1-1.0 mM, activates ADP-phosphate exchange 2fold | Escherichia coli | |
| spermine | - |
Nicotiana tabacum | |
| spermine | 0.1-1.0 mM, activates ADP-phosphate exchange 2fold | Escherichia coli | |
General Stability
| General Stability | Organism |
|---|---|
| highly susceptible to proteolysis | Clostridium perfringens |
| sensitive to proteolytic digestion | Synechococcus elongatus PCC 7942 = FACHB-805 |
| sensitive to proteolytic digestion | Cavia porcellus |
| sensitive to proteolytic digestion | Thermus aquaticus |
| sensitive to proteolytic digestion | Sinorhizobium meliloti |
| sensitive to proteolytic digestion | Escherichia coli |
| sensitive to proteolytic digestion | Rattus norvegicus |
| sensitive to proteolytic digestion | Geobacillus stearothermophilus |
| sensitive to proteolytic digestion | Nicotiana tabacum |
| sensitive to proteolytic digestion | Halobacterium salinarum |
| sensitive to proteolytic digestion | Enterococcus faecalis |
| sensitive to proteolytic digestion | Streptococcus pyogenes |
| sensitive to proteolytic digestion | Achromobacter sp. |
| sensitive to proteolytic digestion | Micrococcus luteus |
| sensitive to proteolytic digestion | Azotobacter vinelandii |
| sensitive to proteolytic digestion | Rhodospirillum rubrum |
| sensitive to proteolytic digestion | Brevibacterium sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-Fluorouridine diphosphate | - |
Achromobacter sp. | |
| 5-Fluorouridine diphosphate | - |
Azotobacter vinelandii | |
| 5-Fluorouridine diphosphate | - |
Brevibacterium sp. | |
| 5-Fluorouridine diphosphate | - |
Cavia porcellus | |
| 5-Fluorouridine diphosphate | - |
Enterococcus faecalis | |
| 5-Fluorouridine diphosphate | - |
Escherichia coli | |
| 5-Fluorouridine diphosphate | - |
Geobacillus stearothermophilus | |
| 5-Fluorouridine diphosphate | - |
Halobacterium salinarum | |
| 5-Fluorouridine diphosphate | - |
Micrococcus luteus | |
| 5-Fluorouridine diphosphate | - |
Nicotiana tabacum | |
| 5-Fluorouridine diphosphate | - |
Rattus norvegicus | |
| 5-Fluorouridine diphosphate | - |
Rhodospirillum rubrum | |
| 5-Fluorouridine diphosphate | - |
Sinorhizobium meliloti | |
| 5-Fluorouridine diphosphate | - |
Streptococcus pyogenes | |
| 5-Fluorouridine diphosphate | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 5-Fluorouridine diphosphate | - |
Thermus aquaticus | |
| 6-azauridine | - |
Achromobacter sp. | |
| 6-azauridine | - |
Azotobacter vinelandii | |
| 6-azauridine | - |
Brevibacterium sp. | |
| 6-azauridine | - |
Cavia porcellus | |
| 6-azauridine | - |
Enterococcus faecalis | |
| 6-azauridine | - |
Escherichia coli | |
| 6-azauridine | - |
Geobacillus stearothermophilus | |
| 6-azauridine | - |
Halobacterium salinarum | |
| 6-azauridine | - |
Micrococcus luteus | |
| 6-azauridine | - |
Nicotiana tabacum | |
| 6-azauridine | - |
Rattus norvegicus | |
| 6-azauridine | - |
Rhodospirillum rubrum | |
| 6-azauridine | - |
Sinorhizobium meliloti | |
| 6-azauridine | - |
Streptococcus pyogenes | |
| 6-azauridine | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 6-azauridine | - |
Thermus aquaticus | |
| Acridine orange | - |
Achromobacter sp. | |
| Acridine orange | - |
Azotobacter vinelandii | |
| Acridine orange | - |
Brevibacterium sp. | |
| Acridine orange | - |
Cavia porcellus | |
| Acridine orange | - |
Enterococcus faecalis | |
| Acridine orange | - |
Escherichia coli | |
| Acridine orange | - |
Geobacillus stearothermophilus | |
| Acridine orange | - |
Halobacterium salinarum | |
| Acridine orange | - |
Micrococcus luteus | |
| Acridine orange | - |
Nicotiana tabacum | |
| Acridine orange | - |
Rattus norvegicus | |
| Acridine orange | - |
Rhodospirillum rubrum | |
| Acridine orange | - |
Sinorhizobium meliloti | |
| Acridine orange | - |
Streptococcus pyogenes | |
| Acridine orange | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Acridine orange | - |
Thermus aquaticus | |
| heparin | - |
Bacillus amyloliquefaciens | |
| phosphonic acid analog of ADP | - |
Achromobacter sp. | |
| phosphonic acid analog of ADP | - |
Azotobacter vinelandii | |
| phosphonic acid analog of ADP | - |
Brevibacterium sp. | |
| phosphonic acid analog of ADP | - |
Cavia porcellus | |
| phosphonic acid analog of ADP | - |
Enterococcus faecalis | |
| phosphonic acid analog of ADP | - |
Escherichia coli | |
| phosphonic acid analog of ADP | - |
Geobacillus stearothermophilus | |
| phosphonic acid analog of ADP | - |
Halobacterium salinarum | |
| phosphonic acid analog of ADP | - |
Micrococcus luteus | |
| phosphonic acid analog of ADP | - |
Nicotiana tabacum | |
| phosphonic acid analog of ADP | - |
Rattus norvegicus | |
| phosphonic acid analog of ADP | - |
Rhodospirillum rubrum | |
| phosphonic acid analog of ADP | - |
Sinorhizobium meliloti | |
| phosphonic acid analog of ADP | - |
Streptococcus pyogenes | |
| phosphonic acid analog of ADP | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
| phosphonic acid analog of ADP | - |
Thermus aquaticus | |
| rifamycin SV | - |
Bacillus amyloliquefaciens | |
| synthetic polynucleotide | - |
Bacillus amyloliquefaciens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Mg2+ | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell membrane | - |
Halobacterium salinarum | - |
- |
| cell membrane | - |
Enterococcus faecalis | - |
- |
| cell membrane | - |
Streptococcus pyogenes | - |
- |
| endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
| membrane | membrane vesicles | Escherichia coli | 16020 | - |
| mitochondrion | - |
Cavia porcellus | 5739 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
| soluble | - |
Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no effect in E. coli enzyme, 0.005 mM, 3fold activation of Bacillus stearothermophilus enzyme | Escherichia coli | |
| Ca2+ | no effect in E. coli enzyme, 0.005 mM, 3fold activation of Bacillus stearothermophilus enzyme | Geobacillus stearothermophilus | |
| Cd2+ | can partially replace Mg2+ in activation | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Cd2+ | can partially replace Mg2+ in activation | Cavia porcellus | |
| Cd2+ | can partially replace Mg2+ in activation | Thermus aquaticus | |
| Cd2+ | can partially replace Mg2+ in activation | Sinorhizobium meliloti | |
| Cd2+ | can partially replace Mg2+ in activation | Escherichia coli | |
| Cd2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
| Cd2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
| Cd2+ | can partially replace Mg2+ in activation | Nicotiana tabacum | |
| Cd2+ | can partially replace Mg2+ in activation | Halobacterium salinarum | |
| Cd2+ | can partially replace Mg2+ in activation | Enterococcus faecalis | |
| Cd2+ | can partially replace Mg2+ in activation | Streptococcus pyogenes | |
| Cd2+ | can partially replace Mg2+ in activation | Achromobacter sp. | |
| Cd2+ | can partially replace Mg2+ in activation | Micrococcus luteus | |
| Cd2+ | can partially replace Mg2+ in activation | Azotobacter vinelandii | |
| Cd2+ | can partially replace Mg2+ in activation | Rhodospirillum rubrum | |
| Cd2+ | can partially replace Mg2+ in activation | Brevibacterium sp. | |
| Co2+ | can partially replace Mg2+ in activation | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Co2+ | can partially replace Mg2+ in activation | Cavia porcellus | |
| Co2+ | can partially replace Mg2+ in activation | Thermus aquaticus | |
| Co2+ | can partially replace Mg2+ in activation | Sinorhizobium meliloti | |
| Co2+ | can partially replace Mg2+ in activation | Escherichia coli | |
| Co2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
| Co2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
| Co2+ | can partially replace Mg2+ in activation | Nicotiana tabacum | |
| Co2+ | can partially replace Mg2+ in activation | Halobacterium salinarum | |
| Co2+ | can partially replace Mg2+ in activation | Enterococcus faecalis | |
| Co2+ | can partially replace Mg2+ in activation | Streptococcus pyogenes | |
| Co2+ | can partially replace Mg2+ in activation | Achromobacter sp. | |
| Co2+ | can partially replace Mg2+ in activation | Micrococcus luteus | |
| Co2+ | can partially replace Mg2+ in activation | Azotobacter vinelandii | |
| Co2+ | can partially replace Mg2+ in activation | Rhodospirillum rubrum | |
| Co2+ | can partially replace Mg2+ in activation | Brevibacterium sp. | |
| Cu2+ | can partially replace Mg2+ in activation | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Cu2+ | can partially replace Mg2+ in activation | Cavia porcellus | |
| Cu2+ | can partially replace Mg2+ in activation | Thermus aquaticus | |
| Cu2+ | can partially replace Mg2+ in activation | Sinorhizobium meliloti | |
| Cu2+ | can partially replace Mg2+ in activation | Escherichia coli | |
| Cu2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
| Cu2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
| Cu2+ | can partially replace Mg2+ in activation | Nicotiana tabacum | |
| Cu2+ | can partially replace Mg2+ in activation | Halobacterium salinarum | |
| Cu2+ | can partially replace Mg2+ in activation | Enterococcus faecalis | |
| Cu2+ | can partially replace Mg2+ in activation | Streptococcus pyogenes | |
| Cu2+ | can partially replace Mg2+ in activation | Achromobacter sp. | |
| Cu2+ | can partially replace Mg2+ in activation | Micrococcus luteus | |
| Cu2+ | can partially replace Mg2+ in activation | Azotobacter vinelandii | |
| Cu2+ | can partially replace Mg2+ in activation | Rhodospirillum rubrum | |
| Cu2+ | can partially replace Mg2+ in activation | Brevibacterium sp. | |
| K+ | - |
Nicotiana tabacum | |
| K+ | potassium salts activate | Micrococcus luteus | |
| K+ | activates polymerization | Micrococcus luteus | |
| Lithium salts | activate | Micrococcus luteus | |
| Mg2+ | required for activity | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Mg2+ | required for activity | Cavia porcellus | |
| Mg2+ | required for activity | Thermus aquaticus | |
| Mg2+ | required for activity | Sinorhizobium meliloti | |
| Mg2+ | required for activity | Escherichia coli | |
| Mg2+ | required for activity | Rattus norvegicus | |
| Mg2+ | required for activity | Geobacillus stearothermophilus | |
| Mg2+ | required for activity | Nicotiana tabacum | |
| Mg2+ | required for activity | Halobacterium salinarum | |
| Mg2+ | required for activity | Enterococcus faecalis | |
| Mg2+ | required for activity | Streptococcus pyogenes | |
| Mg2+ | required for activity | Achromobacter sp. | |
| Mg2+ | required for activity | Micrococcus luteus | |
| Mg2+ | required for activity | Azotobacter vinelandii | |
| Mg2+ | required for activity | Bacillus amyloliquefaciens | |
| Mg2+ | required for activity | Rhodospirillum rubrum | |
| Mg2+ | required for activity | Brevibacterium sp. | |
| Mg2+ | Km: 0.05 mM | Escherichia coli | |
| Mg2+ | 100000 Da form requires high Mg2+ concentrations | Escherichia coli | |
| Mn2+ | can partially replace Mg2+ in activation | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Mn2+ | can partially replace Mg2+ in activation | Cavia porcellus | |
| Mn2+ | can partially replace Mg2+ in activation | Thermus aquaticus | |
| Mn2+ | can partially replace Mg2+ in activation | Sinorhizobium meliloti | |
| Mn2+ | can partially replace Mg2+ in activation | Escherichia coli | |
| Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
| Mn2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
| Mn2+ | can partially replace Mg2+ in activation | Nicotiana tabacum | |
| Mn2+ | can partially replace Mg2+ in activation | Halobacterium salinarum | |
| Mn2+ | can partially replace Mg2+ in activation | Enterococcus faecalis | |
| Mn2+ | can partially replace Mg2+ in activation | Streptococcus pyogenes | |
| Mn2+ | can partially replace Mg2+ in activation | Achromobacter sp. | |
| Mn2+ | can partially replace Mg2+ in activation | Micrococcus luteus | |
| Mn2+ | can partially replace Mg2+ in activation | Azotobacter vinelandii | |
| Mn2+ | can partially replace Mg2+ in activation | Bacillus amyloliquefaciens | |
| Mn2+ | can partially replace Mg2+ in activation | Rhodospirillum rubrum | |
| Mn2+ | can partially replace Mg2+ in activation | Brevibacterium sp. | |
| Mn2+ | 200000 Da form requires Mn2+ for NDP polymerization, polymerization of GDP proceedes efficiently in presence of Mn2+ at 60°C, polymerization with a mutant enzyme from E. coli Q13 requires Mn2+ rather than Mg2+ | Escherichia coli | |
| Mn2+ | stimulates polymerization more efficiently than Mg2+ | Achromobacter sp. | |
| Na+ | activates polymerization | Micrococcus luteus | |
| Na+ | sodium salts activate | Micrococcus luteus | |
| Ni2+ | can partially replace Mg2+ in activation | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Ni2+ | can partially replace Mg2+ in activation | Cavia porcellus | |
| Ni2+ | can partially replace Mg2+ in activation | Thermus aquaticus | |
| Ni2+ | can partially replace Mg2+ in activation | Sinorhizobium meliloti | |
| Ni2+ | can partially replace Mg2+ in activation | Escherichia coli | |
| Ni2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
| Ni2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
| Ni2+ | can partially replace Mg2+ in activation | Nicotiana tabacum | |
| Ni2+ | can partially replace Mg2+ in activation | Halobacterium salinarum | |
| Ni2+ | can partially replace Mg2+ in activation | Enterococcus faecalis | |
| Ni2+ | can partially replace Mg2+ in activation | Streptococcus pyogenes | |
| Ni2+ | can partially replace Mg2+ in activation | Achromobacter sp. | |
| Ni2+ | can partially replace Mg2+ in activation | Micrococcus luteus | |
| Ni2+ | can partially replace Mg2+ in activation | Azotobacter vinelandii | |
| Ni2+ | can partially replace Mg2+ in activation | Rhodospirillum rubrum | |
| Ni2+ | can partially replace Mg2+ in activation | Brevibacterium sp. | |
| Zn2+ | can partially replace Mg2+ in activation | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Zn2+ | can partially replace Mg2+ in activation | Cavia porcellus | |
| Zn2+ | can partially replace Mg2+ in activation | Thermus aquaticus | |
| Zn2+ | can partially replace Mg2+ in activation | Sinorhizobium meliloti | |
| Zn2+ | can partially replace Mg2+ in activation | Escherichia coli | |
| Zn2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
| Zn2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
| Zn2+ | can partially replace Mg2+ in activation | Nicotiana tabacum | |
| Zn2+ | can partially replace Mg2+ in activation | Halobacterium salinarum | |
| Zn2+ | can partially replace Mg2+ in activation | Enterococcus faecalis | |
| Zn2+ | can partially replace Mg2+ in activation | Streptococcus pyogenes | |
| Zn2+ | can partially replace Mg2+ in activation | Achromobacter sp. | |
| Zn2+ | can partially replace Mg2+ in activation | Micrococcus luteus | |
| Zn2+ | can partially replace Mg2+ in activation | Azotobacter vinelandii | |
| Zn2+ | can partially replace Mg2+ in activation | Rhodospirillum rubrum | |
| Zn2+ | can partially replace Mg2+ in activation | Brevibacterium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
alpha3,beta2 or alpha3,betan, x * 86000 + x * 48000, enzyme form B is obtained by keeping the ionic strength at 200 mM during purification on Sephadex G-200, at lower salt concentrations the beta subunit tends to dissociate and the enzyme reverts to the A form | Escherichia coli |
| 51000 | - |
4 * 51000 | Geobacillus stearothermophilus |
| 76000 | - |
2 * 76000, SDS-PAGE | Rhodospirillum rubrum |
| 86000 | - |
alpha3,beta2 or alpha3,betan, x * 86000 + x * 48000, enzyme form B is obtained by keeping the ionic strength at 200 mM during purification on Sephadex G-200, at lower salt concentrations the beta subunit tends to dissociate and the enzyme reverts to the A form | Escherichia coli |
| 100000 | - |
low molecular weight form catalyzing phosphorolysis but unable to catalyze the polymerization of NDP's, can only phosphorolyze short-chain polymers and requires higher Mg2+ ion concentration | Escherichia coli |
| 160000 | - |
gel filtration | Rhodospirillum rubrum |
| 200000 | - |
- |
Azotobacter vinelandii |
| 200000 | - |
this form requires Mn2+ for NDP polymerization and has a higher Km for poly(A) phosphorolysis | Escherichia coli |
| 237000 | - |
sedimentation equilibrium | Micrococcus luteus |
| 252000 | - |
enzyme form A | Escherichia coli |
| 365000 | - |
enzyme form B | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter sp. | - |
KR. 170-4 | - |
| Achromobacter sp. KR. 170-4 | - |
KR. 170-4 | - |
| Azotobacter vinelandii | - |
- |
- |
| Bacillus amyloliquefaciens | - |
BaM-2 | - |
| Bacillus amyloliquefaciens BaM-2 | - |
BaM-2 | - |
| Brevibacterium sp. | - |
- |
- |
| Cavia porcellus | - |
- |
- |
| Clostridium perfringens | - |
- |
- |
| Enterococcus faecalis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Halobacterium salinarum | - |
- |
- |
| Micrococcus luteus | - |
- |
- |
| Nicotiana tabacum | - |
tobacco mosaic virus-infected | - |
| Rattus norvegicus | - |
- |
- |
| Rhodospirillum rubrum | - |
- |
- |
| Sinorhizobium meliloti | - |
- |
- |
| Streptococcus pyogenes | - |
- |
- |
| Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
| Thermus aquaticus | - |
- |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| after heating at 100°C for 1 min 25-30% of the original activity can be recovered by dissolving the precipitate in 6 M guanidine-HCl followed by dialysis | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Nicotiana tabacum | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(A) + ADP | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Cavia porcellus | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Thermus aquaticus | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Sinorhizobium meliloti | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Escherichia coli | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Geobacillus stearothermophilus | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Nicotiana tabacum | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Halobacterium salinarum | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Enterococcus faecalis | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Streptococcus pyogenes | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Achromobacter sp. | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Micrococcus luteus | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Azotobacter vinelandii | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Bacillus amyloliquefaciens | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Rhodospirillum rubrum | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Brevibacterium sp. | poly(A)+1 + phosphate | - |
r | |
| poly(A) + ADP | - |
Bacillus amyloliquefaciens BaM-2 | poly(A)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Cavia porcellus | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Thermus aquaticus | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Sinorhizobium meliloti | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Escherichia coli | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Geobacillus stearothermophilus | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Nicotiana tabacum | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Halobacterium salinarum | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Enterococcus faecalis | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Streptococcus pyogenes | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Achromobacter sp. | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Micrococcus luteus | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Azotobacter vinelandii | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Rhodospirillum rubrum | poly(C)+1 + phosphate | - |
r | |
| poly(C) + CDP | - |
Brevibacterium sp. | poly(C)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Cavia porcellus | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Thermus aquaticus | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Sinorhizobium meliloti | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Escherichia coli | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Geobacillus stearothermophilus | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Nicotiana tabacum | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Halobacterium salinarum | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Enterococcus faecalis | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Streptococcus pyogenes | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Achromobacter sp. | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Micrococcus luteus | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Azotobacter vinelandii | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Bacillus amyloliquefaciens | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Rhodospirillum rubrum | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Brevibacterium sp. | poly(G)+1 + phosphate | - |
r | |
| poly(G) + GDP | - |
Bacillus amyloliquefaciens BaM-2 | poly(G)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Cavia porcellus | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Thermus aquaticus | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Sinorhizobium meliloti | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Escherichia coli | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Geobacillus stearothermophilus | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Nicotiana tabacum | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Halobacterium salinarum | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Enterococcus faecalis | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Streptococcus pyogenes | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Achromobacter sp. | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Micrococcus luteus | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Azotobacter vinelandii | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Rhodospirillum rubrum | poly(I)+1 + phosphate | - |
r | |
| poly(I) + IDP | - |
Brevibacterium sp. | poly(I)+1 + phosphate | - |
r | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Cavia porcellus | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Thermus aquaticus | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Sinorhizobium meliloti | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Escherichia coli | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Rattus norvegicus | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Geobacillus stearothermophilus | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Nicotiana tabacum | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Halobacterium salinarum | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Enterococcus faecalis | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Streptococcus pyogenes | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Achromobacter sp. | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Micrococcus luteus | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Azotobacter vinelandii | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Bacillus amyloliquefaciens | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Rhodospirillum rubrum | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Brevibacterium sp. | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| ribonucleoside 5'-diphosphate + phosphate | - |
Bacillus amyloliquefaciens BaM-2 | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| RNAn + a nucleoside diphosphate | specificity overview | Synechococcus elongatus PCC 7942 = FACHB-805 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Cavia porcellus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | specificity overview | Thermus aquaticus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Escherichia coli | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Geobacillus stearothermophilus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Nicotiana tabacum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Halobacterium salinarum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Enterococcus faecalis | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Streptococcus pyogenes | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Achromobacter sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Micrococcus luteus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Azotobacter vinelandii | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Brevibacterium sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Synechococcus elongatus PCC 7942 = FACHB-805 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Cavia porcellus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Thermus aquaticus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Escherichia coli | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Rattus norvegicus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Geobacillus stearothermophilus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Nicotiana tabacum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Halobacterium salinarum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Enterococcus faecalis | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Streptococcus pyogenes | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Achromobacter sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Micrococcus luteus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Azotobacter vinelandii | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Brevibacterium sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Synechococcus elongatus PCC 7942 = FACHB-805 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Cavia porcellus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Thermus aquaticus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Escherichia coli | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Rattus norvegicus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Geobacillus stearothermophilus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Nicotiana tabacum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Halobacterium salinarum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Enterococcus faecalis | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Streptococcus pyogenes | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Achromobacter sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Micrococcus luteus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Azotobacter vinelandii | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Brevibacterium sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Synechococcus elongatus PCC 7942 = FACHB-805 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Cavia porcellus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Thermus aquaticus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Escherichia coli | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Rattus norvegicus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Geobacillus stearothermophilus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Nicotiana tabacum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Halobacterium salinarum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Enterococcus faecalis | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Streptococcus pyogenes | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Achromobacter sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Micrococcus luteus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Azotobacter vinelandii | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Bacillus amyloliquefaciens | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Brevibacterium sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Synechococcus elongatus PCC 7942 = FACHB-805 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Cavia porcellus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Thermus aquaticus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Escherichia coli | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Rattus norvegicus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Geobacillus stearothermophilus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Nicotiana tabacum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Halobacterium salinarum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Enterococcus faecalis | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Streptococcus pyogenes | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Achromobacter sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Micrococcus luteus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Azotobacter vinelandii | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Bacillus amyloliquefaciens | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Brevibacterium sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Synechococcus elongatus PCC 7942 = FACHB-805 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Cavia porcellus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Thermus aquaticus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Sinorhizobium meliloti | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Escherichia coli | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Rattus norvegicus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Geobacillus stearothermophilus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Nicotiana tabacum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Halobacterium salinarum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Enterococcus faecalis | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Streptococcus pyogenes | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Achromobacter sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Micrococcus luteus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Azotobacter vinelandii | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Rhodospirillum rubrum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | de novo synthesis of polynucleotides, each of the 4 common ribonucleoside diphosphates can serve separately as a substrate for the polymerization reaction, leading to the formation of homopolymers, polymerization of a mixture of nucleoside diphosphates containing different bases results in the formation of a random copolymer, the enzyme does not require a template and cannot copy one, elongation of a primer oligonucleotide with at least 2 nucleoside residues and a free 3'-terminal hydroxyl group, in the reverse reaction breakdown of polyribonucleotides by phosphorolytic cleavage of the internucleotide bonds | Brevibacterium sp. | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Bacillus amyloliquefaciens BaM-2 | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Bacillus amyloliquefaciens BaM-2 | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | specificity overview | Achromobacter sp. KR. 170-4 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of IDP | Achromobacter sp. KR. 170-4 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of CDP | Achromobacter sp. KR. 170-4 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of GDP | Achromobacter sp. KR. 170-4 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Achromobacter sp. KR. 170-4 | RNAn+1 + phosphate | - |
r | |
| RNAn+1 + phosphate | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Cavia porcellus | RNAn + a nucleoside diphosphate | - |
ir | |
| RNAn+1 + phosphate | - |
Thermus aquaticus | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Sinorhizobium meliloti | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Escherichia coli | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Rattus norvegicus | RNAn + a nucleoside diphosphate | - |
ir | |
| RNAn+1 + phosphate | - |
Geobacillus stearothermophilus | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Nicotiana tabacum | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Halobacterium salinarum | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Enterococcus faecalis | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Streptococcus pyogenes | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Achromobacter sp. | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Micrococcus luteus | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Azotobacter vinelandii | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Rhodospirillum rubrum | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Brevibacterium sp. | RNAn + a nucleoside diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Micrococcus luteus |
| ? | alpha3,beta2 or alpha3,betan, x * 86000 + x * 48000, enzyme form B is obtained by keeping the ionic strength at 200 mM during purification on Sephadex G-200, at lower salt concentrations the beta subunit tends to dissociate and the enzyme reverts to the A form | Escherichia coli |
| dimer | 2 * 76000, SDS-PAGE | Rhodospirillum rubrum |
| tetramer | - |
Thermus aquaticus |
| tetramer | 4 * 51000 | Geobacillus stearothermophilus |
| trimer | - |
Micrococcus luteus |
| trimer | alpha3, 3 * 84000-95000, enzyme form A, ultrastructural observations | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Synechococcus elongatus PCC 7942 = FACHB-805 |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Cavia porcellus |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Thermus aquaticus |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Sinorhizobium meliloti |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Escherichia coli |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Rattus norvegicus |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Geobacillus stearothermophilus |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Nicotiana tabacum |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Halobacterium salinarum |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Enterococcus faecalis |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Streptococcus pyogenes |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Achromobacter sp. |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Micrococcus luteus |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Azotobacter vinelandii |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Rhodospirillum rubrum |
| additional information | - |
stabilized against heat inactivation by the presence of NDP's but not by NMP's, NTP's, DNA or substrate oligonucleotides with free 3'-OH termini | Brevibacterium sp. |
| 55 | - |
unstable above | Escherichia coli |
| 65 | - |
- |
Thermus aquaticus |
| 65 | - |
rapid and irreversible inactivation | Escherichia coli |
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Release 2023.1 (January 2023)
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