Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba castellanii | J9XZJ1 | - |
- |
Acanthamoeba castellanii | M1ZML3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mt-tRNALeuDelta+2 + GTP | substrate mimics the presumed in vivo substrate for 5'-end repair, in which the two 5'-mismatched nucleotides have been removed and the resulting tRNA transcript begins with G+3. TLP1 and TLP2 catalyse robust addition of the missing 5'-G nucleotides to this substrate | Acanthamoeba castellanii | ? + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TLP1 | - |
Acanthamoeba castellanii |
Tlp2 | - |
Acanthamoeba castellanii |
General Information | Comment | Organism |
---|---|---|
physiological function | both isoforms TLP1 and TLP2 lack the ability to catalyze efficient G1 addition to cytosolic tRNAHis in vitro and catalyse template-dependent 3'-5' polymerase activity. Both TLP1 and TLP2 exhibit tRNA repair of 5'-edited mt-tRNAs | Acanthamoeba castellanii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
mt-tRNALeuDelta+2 | pH 7.5, 22°C | Acanthamoeba castellanii |