Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L12T/G14A | Km (GTP) increased compared to wild-type, kcat slightly increased compared to wild-type, mutant shows 11-19 fold increased KD (GTP) dissociation constant compared to wild-type | Escherichia coli |
L12T/G14A/P79L/A81T/G82S | Km (GTP) 7-10fold increased compared to wild-type, kcat 3fold decreased compared to wild-type,mutant shows 11-19 fold increased KD (GTP) dissociation constant compared to wild-type | Escherichia coli |
additional information | hybrid protein consisting of N-terminal MobA protein and C-terminal MocA protein shows similar Km (GTP) compared to wild-type, and increased kcat compared to wild-type, mutant shows comparable KD (GTP) dissociation constant compared to wild-type. Compared to wild-type Apo-TorA and TorD only interact with hybrid proteins that contain the C terminus of MobA, but no interaction is identified with variants containing the C terminus of MocA | Escherichia coli |
additional information | hybrid protein consisting of N-terminal MocA protein and C-terminal MobA protein does not show any reactivity towards substrate GTP. Compared to wild-type Apo-TorA and TorD only interact with hybrid proteins that contain the C terminus of MobA, but no interaction is identified with variants containing the C terminus of MocA | Escherichia coli |
P79L/A81T/G82S | Km (GTP) increased compared to wild-type, kcat slightly decreased compared to wild-type, mutant shows 11-19 fold increased KD (GTP) dissociation constant compared to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
GTP | pH 7.2, temperature not specified in the publication, wild-type | Escherichia coli | |
0.0455 | - |
GTP | pH 7.2, temperature not specified in the publication, mutant L12T/G14A/P79L/A81T/G82S | Escherichia coli | |
0.0489 | - |
GTP | pH 7.2, temperature not specified in the publication, mutant L12T/G14A | Escherichia coli | |
0.0706 | - |
GTP | pH 7.2, temperature not specified in the publication, mutant P79L/A81T/G82S | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P32173 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + molybdenum cofactor | - |
Escherichia coli | diphosphate + guanylyl molybdenum cofactor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MobA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
GTP | pH 7.2, temperature not specified in the publication, mutant L12T/G14A/P79L/A81T/G82S | Escherichia coli | |
0.0041 | - |
GTP | pH 7.2, temperature not specified in the publication, mutant P79L/A81T/G82S | Escherichia coli | |
0.0048 | - |
GTP | pH 7.2, temperature not specified in the publication, wild-type | Escherichia coli | |
0.0053 | - |
GTP | pH 7.2, temperature not specified in the publication, mutant L12T/G14A | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Escherichia coli |