Literature summary for 2.7.7.74 extracted from

Kurnasov, O.; Luk, H.; Roberts, M.; Stec, B.
Structure of the inositol-1-phosphate cytidylyltransferase from Thermotoga maritima (2013), Acta Crystallogr. Sect. D, 69, 1808-1817.

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure shows a conserved motif of sugar nucleotide transferases (COG1213) with a structurally reinforced C-terminal Cys bonded to the core of the protein. During catalysis, the conserved Asp107 serves as an electrostatic anchor to which the metal ion (most likely Mg2+) binds. Upon binding, the triphosphate group of the nucleotide interacts with a metal ion as well as the phosphate of the inositol. The O atom of the inositol phosphate group that is polarized by this interaction attacks the triphosphate and causes its cleavage to release the diphosphate group. Lys24 stabilizes the developing negative charge in the transition state and Arg14 serves as a release agent	Thermotoga maritima

Crystallization (Comment)

Organism

structure shows a conserved motif of sugar nucleotide transferases (COG1213) with a structurally reinforced C-terminal Cys bonded to the core of the protein. During catalysis, the conserved Asp107 serves as an electrostatic anchor to which the metal ion (most likely Mg2+) binds. Upon binding, the triphosphate group of the nucleotide interacts with a metal ion as well as the phosphate of the inositol. The O atom of the inositol phosphate group that is polarized by this interaction attacks the triphosphate and causes its cleavage to release the diphosphate group. Lys24 stabilizes the developing negative charge in the transition state and Arg14 serves as a release agent

Thermotoga maritima

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	protein may transiently associate with its membrane-embedded partner phospho-DIP synthase	Thermotoga maritima	-	-

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	G4FFF4	-	-
Thermotoga maritima DSM 3109	G4FFF4	-	-

Synonyms

Synonyms	Comment	Organism
Tmari_1425	-	Thermotoga maritima

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Release 2023.1 (January 2023)