Literature summary for 2.7.7.73 extracted from

Xi, J.; Ge, Y.; Kinsland, C.; McLafferty, F.W.; Begley, T.P.
Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex (2001), Proc. Natl. Acad. Sci. USA, 98, 8513-8518.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
C184S	inactive, C184S is equally effective in catalyzing the formation of [ThiS]-COSH as the wild type enzyme. Cys184 is directly involved in the crosslinking of ThiF to ThiS but is not essential for the formation of [ThiS]-COSH	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [sulfur-carrier protein ThiS]	Escherichia coli	-	diphosphate + adenylyl-[sulfur-carrier protein ThiS]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P30138	-	-

Purification (Commentary)

Purification (Comment)	Organism
G-75 Sephadex gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [sulfur-carrier protein ThiS]	-	Escherichia coli	diphosphate + adenylyl-[sulfur-carrier protein ThiS]	-	?

Synonyms

Synonyms	Comment	Organism
ThiF	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

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Release 2023.1 (January 2023)