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Literature summary for 2.7.7.72 extracted from
- On the role of a conserved, potentially helix-breaking residue in the tRNA-binding alpha-helix of archaeal CCA-adding enzymes (2008), RNA, 14, 1284-1289.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| P295A | kcat /Km for C74 addition is 28% of wild-type value, kcat /Km for C75 addition is 33% of wild-type value, kcat /Km for A76 addition is 1% of wild-type value | Archaeoglobus fulgidus |
| P295G | kcat /Km for C74 addition is 94% of wild-type value, kcat /Km for C75 addition is 15% of wild-type value, kcat /Km for A76 addition is 129% of wild-type value | Archaeoglobus fulgidus |
| P295S | kcat /Km for C74 addition is 3% of wild-type value, kcat /Km for C75 addition is 1% of wild-type value, kcat /Km for A76 addition is 1% of wild-type value | Archaeoglobus fulgidus |
| P295T | kcat /Km for C74 addition is 36% of wild-type value, kcat /Km for C75 addition is 67% of wild-type value, kcat /Km for A76 addition is 92% of wild-type value | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
CTP | C74 addition, pH and temperature not specified in the publication, mutant enzyme P295G | Archaeoglobus fulgidus |  |
| 0.01 | - |
CTP | C74 addition, pH and temperature not specified in the publication, wild-type enzyme | Archaeoglobus fulgidus | |
| 0.025 | - |
CTP | C74 addition, pH and temperature not specified in the publication, mutant enzyme P295T | Archaeoglobus fulgidus | |
| 0.025 | - |
CTP | C75 addition, pH and temperature not specified in the publication, mutant enzyme P295T | Archaeoglobus fulgidus | |
| 0.037 | - |
CTP | C74 addition, pH and temperature not specified in the publication, mutant enzyme P295A | Archaeoglobus fulgidus | |
| 0.04 | - |
CTP | C75 addition, pH and temperature not specified in the publication, mutant enzyme P295A | Archaeoglobus fulgidus | |
| 0.09 | - |
CTP | C75 addition, pH and temperature not specified in the publication, mutant enzyme P295G | Archaeoglobus fulgidus | |
| 0.175 | - |
CTP | C75 addition, pH and temperature not specified in the publication, wild-type enzyme | Archaeoglobus fulgidus | |
| 0.2 | - |
CTP | C74 addition, pH and temperature not specified in the publication, mutant enzyme P295SA | Archaeoglobus fulgidus | |
| 0.233 | - |
ATP | A76 addition, pH and temperature not specified in the publication, mutant enzyme P295G | Archaeoglobus fulgidus | |
| 0.3 | - |
ATP | A76 addition, pH and temperature not specified in the publication, wild-type enzyme | Archaeoglobus fulgidus | |
| 0.35 | - |
ATP | A76 addition, pH and temperature not specified in the publication, mutant enzyme P295T | Archaeoglobus fulgidus | |
| 0.65 | - |
CTP | C75 addition, pH and temperature not specified in the publication, mutant enzyme P295SA | Archaeoglobus fulgidus | |
| 7.64 | - |
ATP | A76 addition, pH and temperature not specified in the publication, mutant enzyme P295A | Archaeoglobus fulgidus | |
| 12.5 | - |
ATP | A76 addition, pH and temperature not specified in the publication, mutant enzyme P295SA | Archaeoglobus fulgidus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | O28126 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a tRNA precursor + 2 CTP + ATP | - |
Archaeoglobus fulgidus | a tRNA with a 3' CCA end + 3 diphosphate | - |
? | |
| a tRNA precursor + CTP | C74 addition | Archaeoglobus fulgidus | a tRNA with a 3' cytidine end + diphosphate | - |
? | |
| a tRNA with a 3' CC end + ATP | A76 addition | Archaeoglobus fulgidus | a tRNA with a 3' CCA end + diphosphate | - |
? | |
| a tRNA with a 3' cytidine + CTP | C75 addition | Archaeoglobus fulgidus | a tRNA with a 3' CC end + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CCA-adding enzyme | - |
Archaeoglobus fulgidus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.76 | - |
ATP | A76 addition, pH and temperature not specified in the publication, mutant enzyme P295SA | Archaeoglobus fulgidus | |
| 0.83 | - |
ATP | A76 addition, pH and temperature not specified in the publication, mutant enzyme P295A | Archaeoglobus fulgidus | |
| 1.83 | - |
CTP | C75 addition, pH and temperature not specified in the publication, mutant enzyme P295SA | Archaeoglobus fulgidus | |
| 2.46 | - |
CTP | C75 addition, pH and temperature not specified in the publication, mutant enzyme P295G | Archaeoglobus fulgidus | |
| 2.48 | - |
CTP | C75 addition, pH and temperature not specified in the publication, mutant enzyme P295A | Archaeoglobus fulgidus | |
| 2.55 | - |
ATP | A76 addition, pH and temperature not specified in the publication, mutant enzyme P295G | Archaeoglobus fulgidus | |
| 2.55 | - |
CTP | C74 addition, pH and temperature not specified in the publication, mutant enzyme P295SA | Archaeoglobus fulgidus | |
| 2.56 | - |
ATP | A76 addition, pH and temperature not specified in the publication, wild-type enzyme | Archaeoglobus fulgidus | |
| 2.72 | - |
ATP | A76 addition, pH and temperature not specified in the publication, mutant enzyme P295T | Archaeoglobus fulgidus | |
| 2.93 | - |
CTP | C75 addition, pH and temperature not specified in the publication, mutant enzyme P295T | Archaeoglobus fulgidus | |
| 3.21 | - |
CTP | C75 addition, pH and temperature not specified in the publication, wild-type enzyme | Archaeoglobus fulgidus | |
| 3.43 | - |
CTP | C74 addition, pH and temperature not specified in the publication, mutant enzyme P295G | Archaeoglobus fulgidus | |
| 3.53 | - |
CTP | C74 addition, pH and temperature not specified in the publication, mutant enzyme P295A | Archaeoglobus fulgidus | |
| 3.68 | - |
CTP | C74 addition, pH and temperature not specified in the publication, wild-type enzyme | Archaeoglobus fulgidus | |
| 3.8 | - |
CTP | C74 addition, pH and temperature not specified in the publication, mutant enzyme P295T | Archaeoglobus fulgidus | |
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