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Literature summary for 2.7.7.72 extracted from

  • Yue, D.; Weiner, A.M.; Maizels, N.
    The CCA-adding enzyme has a single active site (1998), J. Biol. Chem., 273, 29693-29700.
    View publication on PubMed
Search for more literature for 2.7.7.72

Protein Variants

Protein Variants Comment Organism
D106A CTP-adding activity is 90% of wild-type level, ATP-adding activities is 14% of wild-type level Saccharolobus shibatae
D106A mutation has no effect on CCA-adding activity Saccharolobus shibatae
D124A mutation has no effect on CCA-adding activity Saccharolobus shibatae
D143A mutation has no effect on CCA-adding activity Saccharolobus shibatae
D215A CTP-adding activity is 16% of wild-type level, ATP-adding activities is 13% of wild-type level Saccharolobus shibatae
D215A mutation has no effect on CCA-adding activity Saccharolobus shibatae
D218A mutation has no effect on CCA-adding activity Saccharolobus shibatae
D53A no activity Saccharolobus shibatae
D53A/D55A no activity Saccharolobus shibatae
D55A no activity Saccharolobus shibatae
D55E no activity Saccharolobus shibatae
delC135 no activity Saccharolobus shibatae
delC30 no activity Saccharolobus shibatae
E104A mutation has no effect on CCA-adding activity Saccharolobus shibatae
E114A mutation has no effect on CCA-adding activity Saccharolobus shibatae
E139A mutation has no effect on CCA-adding activity Saccharolobus shibatae
E144A mutation has no effect on CCA-adding activity Saccharolobus shibatae
E161A mutation has no effect on CCA-adding activity Saccharolobus shibatae
E173A CTP-adding activity is 19% of wild-type level, ATP-adding activities is 7% of wild-type level Saccharolobus shibatae
E173A mutation has no effect on CCA-adding activity Saccharolobus shibatae
E209A mutation has no effect on CCA-adding activity Saccharolobus shibatae
G156D neither CTP- nor ATP-adding activity is significantly affected Saccharolobus shibatae

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae P77978
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a tRNA precursor + 2 CTP + ATP one catalytic center is responsible for addition of both CTP and ATP. As the single active site catalyzes addition of each nucleotide, the growing 3'-end of the tRNA would progressively refold to create a binding pocket for addition of the next nucleotide Saccharolobus shibatae a tRNA with a 3' CCA end + 3 diphosphate
-
 ?
a tRNA precursor + CTP one catalytic center is responsible for addition of both CTP and ATP. As the single active site catalyzes addition of each nucleotide, the growing 3'-end of the tRNA would progressively refold to create a binding pocket for addition of the next nucleotide Saccharolobus shibatae a tRNA with a 3' cytidine end + diphosphate
-
 ?
a tRNA with a 3' CC end + ATP one catalytic center is responsible for addition of both CTP and ATP. As the single active site catalyzes addition of each nucleotide, the growing 3'-end of the tRNA would progressively refold to create a binding pocket for addition of the next nucleotide Saccharolobus shibatae a tRNA with a 3' CCA end + diphosphate
-
 ?
a tRNA with a 3' cytidine + CTP one catalytic center is responsible for addition of both CTP and ATP. As the single active site catalyzes addition of each nucleotide, the growing 3'-end of the tRNA would progressively refold to create a binding pocket for addition of the next nucleotide Saccharolobus shibatae a tRNA with a 3' CC end + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
CCA-adding enzyme
-
 Saccharolobus shibatae

General Information

General Information Comment Organism
physiological function the enzyme synthesizes and repairs the 3'-terminal CCA sequence of tRNA Saccharolobus shibatae