Literature summary for 2.7.7.72 extracted from

Toh, Y.; Numata, T.; Watanabem K.; Takeshita, D.; Nureki, O.; Tomita, K.
Molecular basis for maintenance of fidelity during the CCA-adding reaction by a CCA-adding enzyme (2008), EMBO J., 27, 1944-1952.

Search for more literature for 2.7.7.72

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment)	Organism
cocrystallisation of enzyme complexes with nine distinct tRNA minihelices. All of the binary and ternary complex crystals belong to the space group P4(3)2(1)2, and contain one complex molecule in the asymmetric unit. Crystal structures are solved at 2.5 to 3.05 A resolutions by molecular replacement	Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a tRNA precursor + 2 CTP + ATP	Archaeoglobus fulgidus	-	a tRNA with a 3' CCA end + 3 diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Archaeoglobus fulgidus	O28126	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a tRNA precursor + 2 CTP + ATP	-	Archaeoglobus fulgidus	a tRNA with a 3' CCA end + 3 diphosphate	-	?
a tRNA precursor + 2 CTP + ATP	the 30-region of RNA is proofread, after two nucleotide additions, in the closed, active form of the complex at the AMP incorporation stage. This proofreading is a prerequisite for the maintenance of fidelity for complete CCA synthesis	Archaeoglobus fulgidus	a tRNA with a 3' CCA end + 3 diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
AfCCA	-	Archaeoglobus fulgidus
CCA-adding enzyme	-	Archaeoglobus fulgidus

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Release 2023.1 (January 2023)