Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
cocrystallisation of enzyme complexes with nine distinct tRNA minihelices. All of the binary and ternary complex crystals belong to the space group P4(3)2(1)2, and contain one complex molecule in the asymmetric unit. Crystal structures are solved at 2.5 to 3.05 A resolutions by molecular replacement | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a tRNA precursor + 2 CTP + ATP | Archaeoglobus fulgidus | - |
a tRNA with a 3' CCA end + 3 diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28126 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a tRNA precursor + 2 CTP + ATP | - |
Archaeoglobus fulgidus | a tRNA with a 3' CCA end + 3 diphosphate | - |
? | |
a tRNA precursor + 2 CTP + ATP | the 30-region of RNA is proofread, after two nucleotide additions, in the closed, active form of the complex at the AMP incorporation stage. This proofreading is a prerequisite for the maintenance of fidelity for complete CCA synthesis | Archaeoglobus fulgidus | a tRNA with a 3' CCA end + 3 diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AfCCA | - |
Archaeoglobus fulgidus |
CCA-adding enzyme | - |
Archaeoglobus fulgidus |