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Literature summary for 2.7.7.72 extracted from
- CCA-adding enzymes and poly(A) polymerases are all members of the same nucleotidyltransferase superfamily: characterization of the CCA-adding enzyme from the archaeal hyperthermophile Sulfolobus shibatae (1996), RNA, 2, 895-908.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Saccharolobus shibatae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
ATP | pH 9.0, 70°C, the initial velocity is a linear function of ATP from 0.015 to 0.2 mM, suugesting that the Km for ATP in presence of each UTP, GTP and CTP is much higher than 0.03 mM | Saccharolobus shibatae |  |
| 0.03 | - |
CTP | pH 9.0, 70°C, in presence of 0.1 mM each of UTP, ATP and GTP | Saccharolobus shibatae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, SDS-PAGE | Saccharolobus shibatae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus shibatae | P77978 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus shibatae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a tRNA precursor + 2 CTP + ATP | - |
Saccharolobus shibatae | a tRNA with a 3'CCA end + 3 diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE | Saccharolobus shibatae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP(CTP):tRNA nucleotidyl transferase | - |
Saccharolobus shibatae |
| CCA-adding enzyme | - |
Saccharolobus shibatae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Saccharolobus shibatae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
weak activity at 50°C, no activity at 37°C | Saccharolobus shibatae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Saccharolobus shibatae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | the enzyme exhibits substantial activity over a broad range from pH 7 to 10 | Saccharolobus shibatae |
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