Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Alkalihalobacillus clausii |
expressed in Escherichia coli | Thermus thermophilus |
expressed in Escherichia coli BL21(DE3)/pLysS Rosetta cells | Geobacter sulfurreducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45590 | - |
NTSFII | Geobacter sulfurreducens |
98390 | - |
NTSFIII | Geobacter sulfurreducens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus clausii | Q5WGA1 | - |
- |
Geobacter sulfurreducens | - |
strain ATCC 51573 | - |
Thermus thermophilus | Q72K91 | - |
- |
Purification (Comment) | Organism |
---|---|
purified by a combination of ammonium sulfate fractionation, gel filtration, and hydrophobic interaction chromatography (NTSFII) | Geobacter sulfurreducens |
purified by a combination of anion-exchange chromatography and hydrophobic interaction chromatography | Geobacter sulfurreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tRNA-C-C | - |
Geobacter sulfurreducens | tRNA-C-C-A + diphosphate | - |
? | |
ATP + tRNA-C-C | - |
Alkalihalobacillus clausii | tRNA-C-C-A + diphosphate | - |
? | |
ATP + tRNA-C-C | - |
Thermus thermophilus | tRNA-C-C-A + diphosphate | - |
? | |
CTP + tRNA-C | - |
Geobacter sulfurreducens | tRNA-C-C + diphosphate | - |
? | |
additional information | NTSFIII is inactive with CTP and tRNAAsp-C as substrates | Geobacter sulfurreducens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A-adding tRNA nucleotidyltransferase | - |
Geobacter sulfurreducens |
C-adding tRNA nucleotidyltransferase | - |
Geobacter sulfurreducens |
CCA-adding enzyme | - |
Alkalihalobacillus clausii |
CCA-adding enzyme | - |
Thermus thermophilus |
CCA-adding enzyme/poly(A) polymerase | - |
Alkalihalobacillus clausii |
CCA-adding enzyme/poly(A) polymerase | - |
Thermus thermophilus |
NTSFII | - |
Geobacter sulfurreducens |
NTSFIII | - |
Geobacter sulfurreducens |
TNT | - |
Geobacter sulfurreducens |