Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibition of AMP incorporating activity only, the CMP incorporating activity is much less sensitive | Escherichia coli | |
1,7-phenanthroline | slight inhibition | Escherichia coli | |
2,2,2-Terpyridyl | inhibition of AMP incorporating activity only, the CMP incorporating activity is much less sensitive | Escherichia coli | |
2,2-dipyridyl | at high concentrations, inhibition of AMP incorporating activity only, the CMP incorporating activity is much less sensitive | Escherichia coli | |
bathophenanthroline | inhibition of AMP incorporating activity only, the CMP incorporating activity is much less sensitive | Escherichia coli | |
additional information | no inhibition by EDTA | Escherichia coli | |
Neocuproine | slight inhibition | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51500 | - |
1 * 51500, SDS-PAGE | Escherichia coli |
53000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 11800fold by ammonium sulfate fractionation, anion exchange and hydroxylapatite chromatography, and tRNA affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67.1 | - |
purified enzyme, pH not specified in the publication, 37°C, pooled column fraction | Escherichia coli |
280 | - |
purified enzyme, pH not specified in the publication, 37°C, column peak fraction | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNAX1 + ATP + 2 CTP | only one protein is responsible for both AMP and CMP incorporation | Escherichia coli | tRNAXCCA + 3 diphosphate | - |
r | |
tRNAX1 + ATP + 2 CTP | only one protein is responsible for both AMP and CMP incorporation | Escherichia coli B / ATCC 11303 | tRNAXCCA + 3 diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 51500, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
adenylyl (cytidylyl)-tRNA nucleotidyltransferase | - |
Escherichia coli |
ATP(CTP): tRNA adenylyl (cytidylyl)transferase | - |
Escherichia coli |
tRNA nucleotidyltransferase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | both AMP- and CMP-incorporating activities, isoelectric focusing | - |
5.85 |