BRENDA - Enzyme Database
show all sequences of 2.7.7.70

Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides

Valvano, M.A.; Messner, P.; Kosma, P.; Microbiology 148, 1979-1989 (2002)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
x * 55000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
Escherichia coli
-
ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
-
700026
Escherichia coli
ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
bifunctional D-beta-D-heptose-7-phosphate kinase/D-beta-dheptose-1-phosphate adenylyltransferase. Based on genomic sequence comparisons, bifunctional proteins are predicted to be present in several Gram-negative microorganisms, including Agrobacterium tumefaciens, Buchnera sp., Caulobacter crescentus, Salmonella typhimurium, Salmonella typhi, Vibrio cholerae, Yersinia pestis, Haemophilus influenzae, Helicobacter pylori and Pseudomonas aeruginosa. In contrast, individual genes encoding domains I and II independently are found in Ralstonia eutropha, Neisseria meningitidis and Neisseria gonorrhoeae. In these cases, it is proposed to use the nomenclature hldA and hldC to indicate the individual kinase- and adenylyltransferase-encoding genes, respectively
700026
Escherichia coli
ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 55000, SDS-PAGE
Escherichia coli
More
the N-terminal domain I spans amino acids 1-318 and shares structural features with members of the ribokinase family. The C-terminal domain II, which spans amino acids 344-477, has all the conserved features of the cytidylyltransferase superfamily
Escherichia coli
Synonyms
Synonyms
Commentary
Organism
bifunctional D-beta-D-heptose-7-phosphate kinase/D-beta-dheptose-1-phosphate adenylyltransferase
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
x * 55000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
Escherichia coli
-
ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
-
700026
Escherichia coli
ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
-
-
?
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
bifunctional D-beta-D-heptose-7-phosphate kinase/D-beta-dheptose-1-phosphate adenylyltransferase. Based on genomic sequence comparisons, bifunctional proteins are predicted to be present in several Gram-negative microorganisms, including Agrobacterium tumefaciens, Buchnera sp., Caulobacter crescentus, Salmonella typhimurium, Salmonella typhi, Vibrio cholerae, Yersinia pestis, Haemophilus influenzae, Helicobacter pylori and Pseudomonas aeruginosa. In contrast, individual genes encoding domains I and II independently are found in Ralstonia eutropha, Neisseria meningitidis and Neisseria gonorrhoeae. In these cases, it is proposed to use the nomenclature hldA and hldC to indicate the individual kinase- and adenylyltransferase-encoding genes, respectively
700026
Escherichia coli
ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55000, SDS-PAGE
Escherichia coli
More
the N-terminal domain I spans amino acids 1-318 and shares structural features with members of the ribokinase family. The C-terminal domain II, which spans amino acids 344-477, has all the conserved features of the cytidylyltransferase superfamily
Escherichia coli
Other publictions for EC 2.7.7.70
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696367
Wang
Divergence of biochemical func ...
Bordetella bronchiseptica
Biochemistry
49
1072-1081
2010
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698565
Kneidinger
Biosynthesis pathway of ADP-L- ...
Escherichia coli
J. Bacteriol.
184
363-369
2002
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700026
Valvano
Novel pathways for biosynthesi ...
Escherichia coli
Microbiology
148
1979-1989
2002
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698557
Valvano
The rfaE gene from Escherichia ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
182
488-497
2000
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