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Literature summary for 2.7.7.7 extracted from

  • Castillo-Lizardo, M.; Henneke, G.; Viguera, E.
    Replication slippage of the thermophilic DNA polymerases B and D from the Euryarchaeota Pyrococcus abyssi (2014), Front. Microbiol., 5, 403.
    View publication on PubMedView publication on EuropePMC
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ varying magnesium concentration affects both slippage and overall DNA synthesisof DNA polymerase PolB and PolD. There is almost no synthesis by PolB, PolD or their exo-forms at low magnesium concentrations (0.1–0.5 mM). Synthesis is also inhibited at 15–20 mM. Parental molecules are readily detectable together with heteroduplex molecules at low to medium magnesium concentrations (1–5 mM) Pyrococcus abyssi

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DNA polymerases PolB and PolD slip in vitro on a template that consists of single-stranded DNA (ssDNA) with a hairpin structure flanked by short direct repeats. Pyrococcus abyssi proliferating cell nuclear antigen increases replication fidelity of this template Pyrococcus abyssi ?
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