KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study of dNTP primer-extension opposite a benzo[a]pyrene-N2-dG-adduct with four DNA polymerases, including Sulfolobus solfataricus Dpo4 and Sulfolobus acidocaldarius Dbh. Vmax/Km is similar for correct dCTP insertion with Dpo4 and Dbh. Compared to Dpo4, Dbh misinsertion is slower for dATP (about 20fold), dGTP (about 110fold) and dTTP (about 6fold), due to decreases in Vmax. These findings provide support that Dbh is in the same Y-Family DNA polymerase class as eukaryotic DNA polymerase kappa and bacterial DNA polymerase IV, which accurately bypass N2-dG adducts | Sulfolobus acidocaldarius | |
additional information | - |
additional information | kinetic study of dNTP primer-extension opposite a benzo[a]pyrene-N2-dG-adduct with four DNA polymerases, including Sulfolobus solfataricus Dpo4 and Sulfolobus acidocaldarius Dbh. Vmax/Km is similar for correct dCTP insertion with Dpo4 and Dbh. Compared to Dpo4, Dbh misinsertion is slower for dATP (about 20fold), dGTP (about 110fold) and dTTP (about 6fold), due to decreases in Vmax. These findings provide support that Dbh is in the same Y-Family DNA polymerase class as eukaryotic DNA polymerase kappa and bacterial DNA polymerase IV, which accurately bypass N2-dG adducts | Saccharolobus solfataricus | |
0.65 | - |
dCTP | pH and temperature not specified in the publication | Sulfolobus acidocaldarius | |
0.74 | - |
dATP | pH and temperature not specified in the publication | Sulfolobus acidocaldarius | |
0.77 | - |
dATP | pH and temperature not specified in the publication | Saccharolobus solfataricus | |
0.94 | - |
dTTP | pH and temperature not specified in the publication | Saccharolobus solfataricus | |
1 | - |
dTTP | pH and temperature not specified in the publication | Sulfolobus acidocaldarius | |
1.2 | - |
dGTP | pH and temperature not specified in the publication | Saccharolobus solfataricus | |
1.4 | - |
dGTP | pH and temperature not specified in the publication | Sulfolobus acidocaldarius | |
2.5 | - |
dCTP | pH and temperature not specified in the publication | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Sulfolobus acidocaldarius | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | - |
Sulfolobus acidocaldarius | diphosphate + DNAn+1 | - |
? | |
dATP + DNAn | dNTP insertion opposite a benzo[a]pyrene-N2-dG-adduct | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
dCTP + DNAn | - |
Sulfolobus acidocaldarius | diphosphate + DNAn+1 | - |
? | |
dCTP + DNAn | dNTP insertion opposite a benzo[a]pyrene-N2-dG-adduct | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
dGTP + DNAn | - |
Sulfolobus acidocaldarius | diphosphate + DNAn+1 | - |
? | |
dGTP + DNAn | dNTP insertion opposite a benzo[a]pyrene-N2-dG-adduct | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | - |
Sulfolobus acidocaldarius | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | dNTP insertion opposite a benzo[a]pyrene-N2-dG-adduct | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DBH | - |
Sulfolobus acidocaldarius |
Dpo4 | - |
Saccharolobus solfataricus |