Literature summary for 2.7.7.7 extracted from

Trincao, J.; Johnson, R.E.; Wolfle, W.T.; Escalante, C.R.; Prakash, S.; Prakash, L.; Aggarwal, A.K.
Dpo4 is hindered in extending a G.T mismatch by a reverse wobble (2004), Nat. Struct. Mol. Biol., 11, 457-462.

Crystallization (Commentary)

Crystallization (Comment)	Organism
the structure of the enzyme bound to GT-mispaired primer template in the presence of an incoming nucleotide is solved. As a control the structure of the enzyme bound to a matched A-T base pair at the primer terminus is also determined. The structures offer a basis for the low efficiency of the enzyme in extending a GT mispair: a reverse wobble that deflects the primer 3'-OH away from the incoming nucleotide	Saccharolobus solfataricus

Crystallization (Comment)

Organism

the structure of the enzyme bound to G*T-mispaired primer template in the presence of an incoming nucleotide is solved. As a control the structure of the enzyme bound to a matched A-T base pair at the primer terminus is also determined. The structures offer a basis for the low efficiency of the enzyme in extending a G*T mispair: a reverse wobble that deflects the primer 3'-OH away from the incoming nucleotide

Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97W02	-	-
Saccharolobus solfataricus P2	Q97W02	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	the enzyme is inefficient at extending mispairs opposite a template G or T, which include, a GT mispair expected to conform closely to Watson-Crick geometry. It is hindered in extending a GT mismatch by a reverse wobble	Saccharolobus solfataricus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	the enzyme is inefficient at extending mispairs opposite a template G or T, which include, a GT mispair expected to conform closely to Watson-Crick geometry. It is hindered in extending a GT mismatch by a reverse wobble	Saccharolobus solfataricus P2	diphosphate + DNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
Dpo4	-	Saccharolobus solfataricus
SSO2448	locus name	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
additional information	-	additional information	pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4	Saccharolobus solfataricus