Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
F12A | no detectable incorporation of ribonucleotide triphosphate is observed with the wild-type enzyme, the mutant form F12A efficiently incorporates and extends ribonucleotide triphosphate, even in the absence of Mn2+ ions | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus P2 | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus P2 | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus P1 | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus P1 | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine | diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus P1 | - |
- |
- |
Saccharolobus solfataricus P2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | DNA polymerase pol Y1 exclusively incorporates 8-OH-GTP opposite adenine. DNA polymerase pol Y1 incorporates 2-OH-dATP predominantly opposite guanine and thymine. DNA polymerase pol B1 incorporates 8-OH-GTP opposite adenine and cytosine. DNA polymerase pol B1 incorporates 2-OH-dATP opposite thymine | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exclusively incorporates 8-OH-GTP opposite adenine | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine | Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine | Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | DNA polymerase pol Y1 exclusively incorporates 8-OH-GTP opposite adenine. DNA polymerase pol Y1 incorporates 2-OH-dATP predominantly opposite guanine and thymine. DNA polymerase pol B1 incorporates 8-OH-GTP opposite adenine and cytosine. DNA polymerase pol B1 incorporates 2-OH-dATP opposite thymine | Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exclusively incorporates 8-OH-GTP opposite adenine | Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine | Saccharolobus solfataricus P1 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine | Saccharolobus solfataricus P1 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | DNA polymerase pol Y1 exclusively incorporates 8-OH-GTP opposite adenine. DNA polymerase pol Y1 incorporates 2-OH-dATP predominantly opposite guanine and thymine. DNA polymerase pol B1 incorporates 8-OH-GTP opposite adenine and cytosine. DNA polymerase pol B1 incorporates 2-OH-dATP opposite thymine | Saccharolobus solfataricus P1 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exclusively incorporates 8-OH-GTP opposite adenine | Saccharolobus solfataricus P1 | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA pol B1 | - |
Saccharolobus solfataricus |
DNA pol Y1 | - |
Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis | Saccharolobus solfataricus |