Literature summary for 2.7.7.7 extracted from

Wong, J.H.; Brown, J.A.; Suo, Z.; Blum, P.; Nohmi, T.; Ling, H.
Structural insight into dynamic bypass of the major cisplatin-DNA adduct by Y-family polymerase Dpo4 (2010), EMBO J., 29, 2059-2069.

Search for more literature for 2.7.7.7

Crystallization (Commentary)

Crystallization (Comment)	Organism
three crystal structures of a the enzyme in complex with Pt-GG DNA (1,2-intrastrand covalent linkage, cis-Pt-1,2-d(GpG)) at 2.9, 1.9, and 2.0 A resolution, respectively. The crystallographic snapshots show three stages of lesion bypass: the nucleotide insertions opposite the 3'G (first insertion) and 5'G (second insertion) of Pt-GG, and the primer extension beyond the lesion site	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97W02	-	-
Saccharolobus solfataricus P2	Q97W02	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	the enzyme bypasses Pt-GG DNA (1,2-intrastrand covalent linkage, cis-Pt-1,2-d(GpG)). This is a dynamic process, in which the lesion is converted from an open and angular conformation at the first insertion to a depressed and nearly parallel conformation at the subsequent reaction stages to fit into the active site of Dpo4	Saccharolobus solfataricus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	the enzyme bypasses Pt-GG DNA (1,2-intrastrand covalent linkage, cis-Pt-1,2-d(GpG)). This is a dynamic process, in which the lesion is converted from an open and angular conformation at the first insertion to a depressed and nearly parallel conformation at the subsequent reaction stages to fit into the active site of Dpo4	Saccharolobus solfataricus P2	diphosphate + DNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
Dpo4	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)