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Literature summary for 2.7.7.7 extracted from

  • Arana, M.; Powell, G.; Edwards, L.; Kunkel, T.; Petrovich, R.
    Refolding active human DNA polymerase ny from inclusion bodies (2010), Protein Expr. Purif., 70, 163-171.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
gene POLN, expression of N-terminally His6-tagged enzyme fragment Pol ny-77 in Escherichia coli in inclusion bodies Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77100
-
 N-terminally His6-tagged enzyme fragment Pol ny-77, gel filtration Homo sapiens
100000
-
 full-length Pol ny Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Homo sapiens
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 gene POLN
-

Purification (Commentary)

Purification (Comment) Organism
recombinant refolded N-terminally His6-tagged enzyme fragment Pol ny-77 by gel filtration, dialysis, and heparin affinity chromatography, recombinant soluble His6-tagged enzyme fragment Pol ny-77 from Escherichia coli by nickel affinity chrmatography Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
use of high hydrostatic pressure to refold recombinant N-terminally His6-tagged enzyme fragment Pol ny-77 from inclusion bodies after expression in Escherichia coli, in soluble and active form. The refolded Pol ny fragment has properties comparable to those of the small amount of Pol m that is purified from the soluble fraction, method evaluation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + DNAn with activated calf thymus DNA Homo sapiens diphosphate + DNAn+1
-
 ?
dCTP + DNAn with activated calf thymus DNA Homo sapiens diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn reversibility of the polymerase reaction at the level of the template with diphosphate as leaving group, not with the alternative leaving groups, overview Homo sapiens diphosphate + DNAn+1
-
 ?
dGTP + DNAn with activated calf thymus DNA Homo sapiens diphosphate + DNAn+1
-
 ?
dTTP + DNAn with activated calf thymus DNA Homo sapiens diphosphate + DNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymerase ny
-
 Homo sapiens
Pol ny
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

General Information

General Information Comment Organism
evolution DNA polymerase ny is a conserved family A DNA polymerase Homo sapiens