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Literature summary for 2.7.7.7 extracted from

  • Imamura, M.; Uemori, T.; Kato, I.; Ishino, Y.
    A non-alpha-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus (1995), Biol. Pharm. Bull., 18, 1647-1652.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ddTTP 0.4 mM, about 80% loss of activity Pyrococcus furiosus
additional information no inhibition by aphidicolin (4 mM) Pyrococcus furiosus
N-ethylmaleimide 1 mM, about 80% loss of activity Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimum: 25-150 mM Pyrococcus furiosus
MgCl2 optimal concentration: 10 mM Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Pyrococcus furiosus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus P81409 DNA polymerase II large subunit
-

Purification (Commentary)

Purification (Comment) Organism
partial Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.07
-
75°C, pH not specified in the publication Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Pyrococcus furiosus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme utilizes activated DNA as a template-primer, artificial substrate (activated poly(dA-dT)) is preferred by the enzyme. M13 single-stranded DNA primed with 17 base oligonucleotide is not a good substrate. DNA elongation ability of Pol II using a natural DNA template is much lower than that of Pol I from this organism and DNA synthesis of Pol II seems to be non-processive Pyrococcus furiosus diphosphate + DNAn+1
-
?

Subunits

Subunits Comment Organism
? x * 130000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
Pol II
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
assay at Pyrococcus furiosus
80
-
substrate: calf thymus activated DNA Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 85 70°C: about 55% of maximal activity, 85°C: about 70% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
60 min, about 50% loss of activity Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
7 8.8 pH 7.0: about 75% of maximal activity, pH 8.8: about 75% of maximal activity Pyrococcus furiosus