Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ddTTP | 0.4 mM, about 80% loss of activity | Pyrococcus furiosus | |
additional information | no inhibition by aphidicolin (4 mM) | Pyrococcus furiosus | |
N-ethylmaleimide | 1 mM, about 80% loss of activity | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimum: 25-150 mM | Pyrococcus furiosus | |
MgCl2 | optimal concentration: 10 mM | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Pyrococcus furiosus | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | P81409 | DNA polymerase II large subunit | - |
Purification (Comment) | Organism |
---|---|
partial | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.07 | - |
75°C, pH not specified in the publication | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Pyrococcus furiosus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme utilizes activated DNA as a template-primer, artificial substrate (activated poly(dA-dT)) is preferred by the enzyme. M13 single-stranded DNA primed with 17 base oligonucleotide is not a good substrate. DNA elongation ability of Pol II using a natural DNA template is much lower than that of Pol I from this organism and DNA synthesis of Pol II seems to be non-processive | Pyrococcus furiosus | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 130000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
Pol II | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
assay at | Pyrococcus furiosus |
80 | - |
substrate: calf thymus activated DNA | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 85 | 70°C: about 55% of maximal activity, 85°C: about 70% of maximal activity | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
60 min, about 50% loss of activity | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.8 | pH 7.0: about 75% of maximal activity, pH 8.8: about 75% of maximal activity | Pyrococcus furiosus |