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Literature summary for 2.7.7.7 extracted from

  • Boudsocq, F.; Iwai, S.; Hanaoka, F.; Woodgate, R.
    Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta (2001), Nucleic Acids Res., 29, 4607-4616.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40189
-
x * 40189, calculated from sequence Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P96022
-
-
Saccharolobus solfataricus P2 P96022
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn in the absence of additional cofactor the enzyme is an essentially distributive enzyme that only extends primers by 1-2 nt per binding event. At high enzyme to primer/template ratios, dissociation and rebinding of the enzyme to the primer/template is robust and can lead to the synthesis of polynucleotide chains of several hundred nucleotides in length Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn in the absence of additional cofactor the enzyme is an essentially distributive enzyme that only extends primers by 1-2 nt per binding event. At high enzyme to primer/template ratios, dissociation and rebinding of the enzyme to the primer/template is robust and can lead to the synthesis of polynucleotide chains of several hundred nucleotides in length Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
?

Subunits

Subunits Comment Organism
? x * 40189, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
DNA polymerase IV
-
Saccharolobus solfataricus
Dpo4
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 95 high activity in the temperature range Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
5 min, enzyme is not denatured after heating of crude cell lysate Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus calculated from sequence
-
9.1