Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM | Sulfolobus acidocaldarius | |
KCl | 100 mM, 80% inhibition | Sulfolobus acidocaldarius | |
Mg2+ | above 2 mM | Sulfolobus acidocaldarius | |
additional information | no inhibition by 0.02 mg/ml aphidicolin. The enzyme is inhibited by dideoxy- and arabinosine-analogs and SH-blocking agents | Sulfolobus acidocaldarius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme requires an extremely low concentration of Mg+2 cations for activity. A broad plateau is observed between 0.1 and 2 mM MgCl2. Concentrations above 2 mM are inhibitory. 20% of the activity of the plateau value is still observed even if no MgCl2 is added to the assay | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
1 * 100000, SDS-PAGE | Sulfolobus acidocaldarius |
108000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | P95690 | - |
- |
Sulfolobus acidocaldarius DSM 639 | P95690 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
178 | - |
pH 8.0, 65°C, incorporation of TTP | Sulfolobus acidocaldarius |
Storage Stability | Organism |
---|---|
-20°C, in the presence of 20% glycerol, no substantial loss of activity after several weeks | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | the activated herring sperm DNA is an optimal template and gives 3times higher activity than that obtained with poly(dA)/oligo(dT). The DNA polymerase can not use templates without primer (poly(dA) and poly(dT)), even when appropriate priming ribonucleotides (UTP or ATP, respectively) are supplied. It does not accept a polyribonucleotide template (poly(rA):oligo(dT)) | Sulfolobus acidocaldarius | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the activated herring sperm DNA is an optimal template and gives 3times higher activity than that obtained with poly(dA)/oligo(dT). The DNA polymerase can not use templates without primer (poly(dA) and poly(dT)), even when appropriate priming ribonucleotides (UTP or ATP, respectively) are supplied. It does not accept a polyribonucleotide template (poly(rA):oligo(dT)) | Sulfolobus acidocaldarius DSM 639 | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Sulfolobus acidocaldarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 65 | activity at 65°C (optimum) is 20fold higher than at 37°C. A rapid 10fold increase occurs at 70-80°C | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
maximal activity in 50 mM potassium phosphate buffer | Sulfolobus acidocaldarius |
8 | - |
assay at | Sulfolobus acidocaldarius |
8 | - |
maximal activity in 50 mM Tris-HCl buffer | Sulfolobus acidocaldarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 5.5: about 70% of maximal activity, pH 8.0: about 20% of maximal activity, maximal activity in 50 mM potassium phosphate buffer | Sulfolobus acidocaldarius |
7 | 9.5 | pH 7.0: about 70% of maximal activity, pH 9.5: about 70% of maximal activity, in 50 mM Tris-HCl buffer | Sulfolobus acidocaldarius |