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Literature summary for 2.7.7.7 extracted from
- Thermostable DNA polymerase from the archaebacterium Sulfolobus acidocaldarius. Purification, characterization and immunological properties (1989), Eur. J. Biochem., 178, 619-626.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',3'-dideoxyribosylthymine triphosphate | 17% and 33% inhibition are observed with ddTTP/dTTP ratios of 1:1 and 5:1, respectively | Sulfolobus acidocaldarius |  |
| glycerol | 50% inhibition with 15% glycerol | Sulfolobus acidocaldarius | |
| additional information | the enzyme is resistant to 0.02 mg/ml aphidicolin | Sulfolobus acidocaldarius | |
| N-ethylmaleimide | 5 mM; 66% inhibition | Sulfolobus acidocaldarius | |
| NH4Cl | 150 mM, 50% inhibition | Sulfolobus acidocaldarius | |
| sucrose | 50% inhibition with 4% sucrose | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
1 * 100000, SDS-PAGE | Sulfolobus acidocaldarius |
| 109000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
- |
- |
| Sulfolobus acidocaldarius DSM 639 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 100000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Sulfolobus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 80 | 55°C: about 45% of maximal activity, 80°C: about 45% of maximal activity, substrate activated calf-thymus DNA | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
stable up to | Sulfolobus acidocaldarius |
| 87 | - |
15 min, 50% loss of activity | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 70% of maximal activity, pH 9.0: about 70% of maximal activity | Sulfolobus acidocaldarius |
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