Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
Dpo4 in complex with DNA duplex containing N2,N2-dimethyl-substituted guanine-modified template, sitting drop vapor diffusion method, using 10-15% polyethylene glycol 3350 (w/v), 30 mM NaCl, 100 mM MgCl2, and 3% glycerol (v/v) | Saccharolobus solfataricus |
sitting drop, vapor-diffusion method with the reservoir solution containing 1015% polyethylene glycol 3350 (w/v), 30 mm NaCl, 100 mm MgCl2, and 3% glycerol (v/v). One crystal structure of Dpo4 with a primer having a 3'-terminal dideoxycytosine opposite template N2,N2-dimethylguanine in a post-insertion position shows dideoxycytosine folded back into the minor groove, as a catalytically incompetent complex. A second crystal has two unique orientations for the primer terminal dideoxycytosine as follows: (I) flipped into the minor groove and (II) a long pairing with N2,N2-dimethylguanine in which one hydrogen bond exists between the O-2 atom of dideoxycytosine and the N-1 atom of N2,N2-dimethylguanine, with a second water-mediated hydrogen bond between the N-3 atom of dideoxycytosine and the O-6 atom of N2,N2-dimethylguanine. A crystal structure of Dpo4 with dTTP opposite template N2,N2-dimethylguanine reveals a wobble orientation | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N2,N2-dimethyl guanine | the presence of N2,N2-dimethyl guanine lowers the catalytic efficiency for incorporation of dCTP of DNA polymerase Dpo4 16000fold | Saccharolobus solfataricus | |
N2,N2-dimethylguanine | the presence of N2,N2-dimethylguanine lowers the catalytic efficiency of the DNA polymerase Dpo4 16000fold. Dpo4 inserts dNTPs almost at random during bypass of N2,N2-dimethylguanine, and much of the enzyme is kinetically trapped by an inactive ternary complex when N2,N2-dimethylguanine is present | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97W02 | - |
- |
Saccharolobus solfataricus P2 | Q97W02 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase Dpo4 | - |
Saccharolobus solfataricus |