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Literature summary for 2.7.7.7 extracted from
- Mechanisms of DNA polymerases (2009), Chem. Rev., 109, 2862-2879.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D112A/E114A | the mutant T4 DNA polymerases is defective in 3'-5' exonuclease activity and has a 1000fold increase in the development of spontaneous mutations compared to wild type polymerase | Tequatrovirus T4 |
| D219A | the mutant T4 DNA polymerases is defective in 3'-5' exonuclease activity and has a 1000fold increase in the development of spontaneous mutations compared to wild type polymerase | Tequatrovirus T4 |
| D324A | the mutant T4 DNA polymerases is defective in 3'-5' exonuclease activity and has a 1000fold increase in the development of spontaneous mutations compared to wild type polymerase, the exonuclease activity of the D324A mutant is 100000-fold lower than wild type polymerase | Tequatrovirus T4 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Escherichia phage RB69 | - |
- |
- |
| Escherichia phage T7 | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Tequatrovirus T4 | - |
- |
- |
| Thermus aquaticus | P19821 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Bacillus subtilis | diphosphate + DNAn+1 | - |
? |  |
| deoxynucleoside triphosphate + DNAn | - |
Escherichia coli | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Homo sapiens | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Geobacillus stearothermophilus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Tequatrovirus T4 | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Escherichia phage T7 | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Escherichia phage RB69 | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Thermus aquaticus | diphosphate + DNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA polymerase | - |
Bacillus subtilis |
| DNA polymerase | - |
Escherichia coli |
| DNA polymerase | - |
Geobacillus stearothermophilus |
| DNA polymerase | - |
Tequatrovirus T4 |
| DNA polymerase | - |
Escherichia phage T7 |
| DNA polymerase | - |
Escherichia phage RB69 |
| DNA polymerase | - |
Thermus aquaticus |
| DNA polymerase I | - |
Homo sapiens |
| Pol | - |
Bacillus subtilis |
| Pol | - |
Escherichia coli |
| Pol | - |
Geobacillus stearothermophilus |
| Pol | - |
Tequatrovirus T4 |
| Pol | - |
Escherichia phage T7 |
| Pol | - |
Escherichia phage RB69 |
| Pol | - |
Thermus aquaticus |
| Pol I | - |
Homo sapiens |
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