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Literature summary for 2.7.7.7 extracted from

  • Zhang, H.; Beckman, J.; Wang, J.; Konigsberg, W.
    RB69 DNA polymerase mutants with expanded nascent base-pair-binding pockets are highly efficient but have reduced base selectivity (2009), Biochemistry, 48, 6940-6950.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia phage RB69

Protein Variants

Protein Variants Comment Organism
L561A the mutant has pre-steadystate Kd,app and kpol values for the incorporation of correct dNMPs that are comparable to or exhibits kpol values that are somewhat greater than wild type DNA polymerase Escherichia phage RB69
L561A/Y567A the mutant has pre-steadystate Kd,app and kpol values for the incorporation of correct dNMPs that are comparable to or exhibits kpol values that are somewhat greater than wild type DNA polymerase Escherichia phage RB69
L561A/Y567A/S565G the mutant has pre-steadystate Kd,app and kpol values for the incorporation of correct dNMPs that are comparable to or exhibits kpol values that are somewhat greater than wild type RB69 DNA polymerase Escherichia phage RB69
Y567A the mutant has pre-steadystate Kd,app and kpol values for the incorporation of correct dNMPs that are comparable to or exhibits kpol values that are somewhat greater than wild type DNA polymerase Escherichia phage RB69

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Escherichia phage RB69
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Escherichia phage RB69
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Escherichia phage RB69 diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
DNA polymerase
-
Escherichia phage RB69
Pol
-
Escherichia phage RB69