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Literature summary for 2.7.7.7 extracted from

  • Kukreti, P.; Singh, K.; Ketkar, A.; Modak, M.J.
    Identification of a new motif required for the 3-5 exonuclease activity of Escherichia coli DNA polymerase I (Klenow fragment): the RRRY motif is necessary for the binding of single-stranded DNA substrate and the template strand of the mismatched duplex (2008), J. Biol. Chem., 283, 17979-17990.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
mutants are expressed in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
F771A the mutation shows 30% decreased polymerase activity compared to the wild type enzyme Escherichia coli
L823A the mutation shows 3% decreased polymerase activity compared to the wild type enzyme Escherichia coli
R821A the mutation shows 19% increased polymerase activity compared to the wild type enzyme Escherichia coli
R822A the mutation shows 5% increased polymerase activity compared to the wild type enzyme Escherichia coli
R822A/Y824A the mutation shows 36% increased polymerase activity compared to the wild type enzyme Escherichia coli
Y824A the mutation shows 25% increased polymerase activity compared to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 deoxynucleoside triphosphate wild type enzyme, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C Escherichia coli
0.0015
-
 deoxynucleoside triphosphate mutant enzyme R821A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C Escherichia coli
0.002
-
 deoxynucleoside triphosphate mutant enzyme Y824A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C Escherichia coli
0.0026
-
 deoxynucleoside triphosphate mutant enzyme R823A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C Escherichia coli
0.0034
-
 deoxynucleoside triphosphate mutant enzyme R822A/Y824A in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C Escherichia coli
0.0037
-
 deoxynucleoside triphosphate mutant enzyme R822A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P00582
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Escherichia coli diphosphate + DNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymerase I
-
 Escherichia coli
Klenow fragment the Klenow fragment of Escherichia coli DNA polymerase I houses catalytic centers for both polymerase and 3'-5' exonuclease activities Escherichia coli
Pol I
-
 Escherichia coli