Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | considerable enhancement in processivity at 1 mM ATP | Vigna radiata | |
spermidine | considerable enhancement in processivity at 2-4 mM spermidine | Vigna radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ddTTP | processivity is strongly inhibited in the presence of 0.01 and 0.02 mM ddTTP | Vigna radiata | |
Mg2+ | Mg2+ concentrations beyond 6 mM are inhibitory in activity assay | Vigna radiata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | optimal processivity at 100-150 mM | Vigna radiata | |
Mg2+ | optimal processivity at 6 mM, no synthesis is observed in the absence of Mg2+ | Vigna radiata | |
Mn2+ | - |
Vigna radiata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
SDS-PAGE | Vigna radiata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Vigna radiata | - |
seedling | active in both meristematic and meiotic tissues | Vigna radiata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Vigna radiata | diphosphate + DNAn+1 | - |
? | |
additional information | the enzyme is unable to use single stranded DNA or double stranded blunt end DNA | Vigna radiata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ddNTP-sensitive DNA polymerase | - |
Vigna radiata |
DNA pol | - |
Vigna radiata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Vigna radiata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
activity is almost completely lost above 48°C | Vigna radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Vigna radiata |