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Literature summary for 2.7.7.7 extracted from
- Reconstitution of a minimal DNA replicase from Pseudomonas aeruginosa and stimulation by non-cognate auxiliary factors (2005), J. Biol. Chem., 280, 7890-7900.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | reconstitution of replicase activity by co-expression of essential components of DNA polymerase holoenzyme from Pseudomonas aeruginosa: expression of processivity factor, i.e. beta-subunit, single-stranded DNA-binding protein, a complex containing the polymerase, i.e. alpha-subunit, and exonuclease, i.e. epsilon-subunit, and the essential components of the DnaX complex tau3deltadelta', Pseudomonas aeruginosa alphaepsilon can substitute completely for Escherichia coli polymerase III in Escherichia coli holoenzyme reconstitution assays, addition of purified Escherichia coli chi and psi, components of the DnaX complex, increases the apparent specific activity of the Pseudomonas tau3deltadelta' complex about 10-fold and enables the reconstituted enzyme to function better under physiological salt conditions, overview | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pseudomonas aeruginosa |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | DNA synthesis on M13Gori single-stranded phage DNA as template DNA | Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA polymerase III | - |
Pseudomonas aeruginosa |
| DNA replicase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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