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Literature summary for 2.7.7.7 extracted from
- Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography (2004), Biochem. J., 382, 535-543.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aphidicolin | IC50: 0.01 mM | Drosophila melanogaster |  |
| KCl | 150-200 mM | Drosophila melanogaster | |
| additional information | enzyme is resistant to NEM and 2',3'-dideoxythymidine 5-triphosphate | Drosophila melanogaster |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent cation required, optimal concentration is 5 mm | Drosophila melanogaster | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 240000 | - |
gel filtration | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Drosophila melanogaster | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | preference for poly(dA)/oligo(dT)10:1 as a template primer and has high processivity for DNA synthesis | Drosophila melanogaster | diphosphate + DNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dmpol zeta | - |
Drosophila melanogaster |
| DNA polymerase zeta | - |
Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Drosophila melanogaster |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | about 50% of maximal activity at pH 6.5 and 8.5 | Drosophila melanogaster |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
IC50: 0.01 mM | Drosophila melanogaster | aphidicolin | |
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