Cloned (Comment) | Organism |
---|---|
PI-TfuI and PI-TfuII | Thermococcus fumicolans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | PI-TfuII is inhibited by one of the cleavage products | Thermococcus fumicolans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | PI-TfuI utilizes either Mg2+ or Mn2+, PI-TfuII only utilizes Mg2+ | Thermococcus fumicolans | |
Mn2+ | PI-TfuI utilizes either Mg2+ or Mn2+, PI-TfuII only utilizes Mg2+ | Thermococcus fumicolans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Thermococcus fumicolans | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus fumicolans | P74918 | - |
- |
Purification (Comment) | Organism |
---|---|
PI-TfuI and PI-TfuII | Thermococcus fumicolans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermococcus fumicolans | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | PI-TfuI recognizes a minimal sequence of 16 base pairs, PI-TfuII requires a sequence of 21 base pairs, both enzymes have endonuclease activity | Thermococcus fumicolans | diphosphate + DNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermococcus fumicolans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable | Thermococcus fumicolans |