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Literature summary for 2.7.7.7 extracted from

  • Ma, W.P.; Kaiser, M.W.; Lyamicheva, N.; Schaefer, J.J.; Allawi, H.T.; Takova, T.; Neri, B.P.; Lyamichev, V.I.
    RNA template-dependent 5' nuclease activity of Thermus aquaticus and Thermus thermophilus DNA polymerases (2000), J. Biol. Chem., 275, 24693-24700.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes Thermus aquaticus
wild-type and mutant enzymes Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
G418K increased exonuclease activity Thermus aquaticus
Q507E increased exonuclease activity Thermus aquaticus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ optimal concentration: 50 mM Thermus thermophilus
K+ optimal concentration for wild-type enzyme: 50 mM, optimal concentration for G418/E507Q mutant: 100 mM Thermus aquaticus
Mg2+ optimal concentration: 8 mM Thermus aquaticus
Mg2+ optimal concentration: 8 mM Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Thermus aquaticus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Thermus thermophilus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Thermus aquaticus
wild-type and mutant enzymes Thermus thermophilus

Reaction

Reaction Comment Organism Reaction ID
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 binding mechanism Thermus aquaticus
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 binding mechanism Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Thermus aquaticus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Thermus thermophilus diphosphate + DNAn+1
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0667
-
deoxynucleoside triphosphate wild-type enzyme, pH 7.5, 60°C Thermus aquaticus
0.0667
-
deoxynucleoside triphosphate wild-type enzyme, pH 7.5, 60°C Thermus thermophilus
0.15
-
deoxynucleoside triphosphate G418K/E507Q mutant, pH 7.5, 60°C Thermus aquaticus
0.15
-
deoxynucleoside triphosphate G418K/E507Q mutant, pH 7.5, 60°C Thermus thermophilus