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Literature summary for 2.7.7.7 extracted from
- Purification and properties of the 5'-3' exonuclease D10A mutant of DNA polymerase I from Streptococcus pneumoniae: a new tool for DNA sequencing (1998), J. Biotechnol., 63, 17-27.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and D10A mutant | Streptococcus pneumoniae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D10A | retains polymerase activity, reduced exonuclease activity, changes in dependency on metal activation of exonuclease activity | Streptococcus pneumoniae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | higher polymerase activity with Mg2+ than with Mn2+, exonuclease acitivity shows equal efficiency with Mg2+ and Mn2+ | Streptococcus pneumoniae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 99000 | - |
SDS-PAGE | Streptococcus pneumoniae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Streptococcus pneumoniae | - |
diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.84 | - |
- |
Streptococcus pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Streptococcus pneumoniae | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | exonuclease 5'--3' activity, pol I | Streptococcus pneumoniae | diphosphate + DNAn+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptococcus pneumoniae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 55 | highest activity at 37°C, enzyme is also active at 55°C | Streptococcus pneumoniae |
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