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Literature summary for 2.7.7.7 extracted from
- Purification and properties of DNA polymerase from Bacillus caldotenax (1992), Biochem. J., 287, 971-977.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aphidicolin | 50% inhibition at 0.05 mM | [Bacillus] caldotenax |  |
| Ara-CTP | 50% inhibition at 0.05 mM | [Bacillus] caldotenax | |
| Dideoxythymidine triphosphate | 50% inhibition at 0.25 mM | [Bacillus] caldotenax | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace Mg2+ in activation, optimal concentration: 2.5 mM | [Bacillus] caldotenax | |
| K+ | stimulates at 100 mM | [Bacillus] caldotenax | |
| Mg2+ | stimulates at 70 mM | [Bacillus] caldotenax | |
| Mn2+ | can partially replace Mg2+ in activation | [Bacillus] caldotenax | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
1 * 94000, SDS-PAGE | [Bacillus] caldotenax |
| 110000 | 120000 | HPLC gel filtration, non-denaturing PAGE | [Bacillus] caldotenax |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | [Bacillus] caldotenax | - |
diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Bacillus] caldotenax | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Bacillus] caldotenax |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
[Bacillus] caldotenax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
[Bacillus] caldotenax | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | template specificity | [Bacillus] caldotenax | diphosphate + DNAn+1 | - |
? | |
| dNTP + DNAn | - |
[Bacillus] caldotenax | diphosphate + DNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 94000, SDS-PAGE | [Bacillus] caldotenax |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
[Bacillus] caldotenax |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 55 | 37°C: 15% of activity maximum, 55°C: 65% of activity maximum | [Bacillus] caldotenax |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
t1/2: 1.5 h without glycerol, 20 h in presence of glycerol | [Bacillus] caldotenax |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
dNTP | - |
[Bacillus] caldotenax | |
| 1.05 | - |
deoxynucleoside triphosphate | equimolar mixture of all nucleotides, pH 7.5, 70°C | [Bacillus] caldotenax | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
[Bacillus] caldotenax |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| [Bacillus] caldotenax | PAGE, pH range 3-9, pH 7.5, 70°C | - |
4.9 |
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